Product nameAnti-Repulsive Guidance Molecule C antibody [Fery-1]
See all Repulsive Guidance Molecule C primary antibodies
DescriptionMouse monoclonal [Fery-1] to Repulsive Guidance Molecule C
Specificityab54431 recognizes human Repulsive Guidance Molecule C.
Tested applicationsSuitable for: IP, Flow Cyt, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment, corresponding to amino acids 36-398 of Human Repulsive Guidance Molecule C
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from concentrated hybridoma tissue cell culture supernatant. Greater than 95% pure by SDS-PAGE.
Our Abpromise guarantee covers the use of ab54431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Detects a band of approximately 50, 37, 45, 74, 100 kDa (predicted molecular weight: 50, 37 kDa).|
RelevanceMutations in Repulsive Guidance Molecule (also known as RGM-C; HJV; hemojuvelin; Hfe2) cause juvenile hemochromatosis, a severe iron overload disease. RGM-C gene expression has been characterized in the developing mouse and found to be exclusively expressed in all striated muscle and in the myocardium.
Cellular localizationCell membrane; Lipid-anchor, GPI-anchor
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All lanes : Anti-Repulsive Guidance Molecule C antibody [Fery-1] (ab54431) at 1/1000 dilution
Lane 1 : embryonic day 16 mouse muscle
Lane 2 : adult mouse heart
Lysates/proteins at 20 µg per lane.
All lanes : HRP conjugated at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50, 37 kDa
Additional bands at: 45&74&100 kDa (possible non-specific binding)
Exposure time: 2 minutes
This product has been referenced in:
- van Swelm RP et al. Acute acetaminophen intoxication leads to hepatic iron loading by decreased hepcidin synthesis. Toxicol Sci 129:225-33 (2012). Read more (PubMed: 22610607) »
- Lakhal S et al. Regulation of Type II Transmembrane Serine Proteinase TMPRSS6 by Hypoxia-inducible Factors: NEW LINK BETWEEN HYPOXIA SIGNALING AND IRON HOMEOSTASIS. J Biol Chem 286:4090-7 (2011). Flow Cyt ; Human . Read more (PubMed: 20966077) »