Resazurin Assay Kit - Cell Cytotoxicity (Fluorometric)  (ab112119)

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Functional Studies - Cell Cytotoxicity Assay Kit (Fluorometric) (ab112119)

    Key features and details

    • Assay type: Quantitative
    • Detection method: Fluorescent
    • Platform: Microplate reader
    • Assay time: 1 hr
    • Sample type: Adherent cells, Suspension cells

    Overview

    • Product name

      Resazurin Assay Kit - Cell Cytotoxicity (Fluorometric) 
      See all Cell Cytotoxicity kits

    • Detection method

      Fluorescent

    • Sample type

      Adherent cells, Suspension cells

    • Assay type

      Quantitative

    • Assay time

      1h 00m

    • Species reactivity

      Reacts with: Mammals, Other species

    • Product overview


      Recommended alternative kit
      We recommend that you purchase Resazurin Assay Kit ab129732 instead of this kit.


       


      Resazurin Assay Kit ab112119 (Cell Cytotoxicity, Fluorometric) ab112119 provides a fast, simple, accurate and homogeneous assay for the fluorometric detection of viable cells.


      The resazurin assay protocol is based on the reduction of oxidized non-fluorescent blue resazurin to a red fluorescent dye (resorufin) by the mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells. Resorufin has Ex/Em of 530-560 / 590 nm.


      The resazurin assay is very commonly used to quantify the numbers of live cells, and monitor cell viability / cytotoxicity.


      ab112119 is more sensitive for cell proliferation and cytotoxicity than other assays such as MTT. The kit components are stable and have minimal cytotoxicity, thus a longer incubation time (such as 24-48 hours) is possible if required.


      Resazurin assay protocol summary:
      - add assay solution to cells in 96 or 384 well plates and shake gently for 30 sec
      - incubate for 1-24 hr at 37ºC
      - analyze with microplate reader

    • Notes

      ab112119 should be stored desiccated

    • Platform

      Microplate reader

    Properties

    Images

    • Functional Studies - Cell Cytotoxicity Assay Kit (Fluorometric) (ab112119)
      Functional Studies - Cell Cytotoxicity Assay Kit (Fluorometric) (ab112119)
      CHO-K1 cell number response was measured with ab112119. CHO-K1 cells at 0 to 10,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 20 µL/well of Assay Solution for 3 hours at 37 °C. The absorbance intensity was measured at 570 nm and 605 nm. The fluorescence intensity was linear (R2 = 0.998) to the cell number as indicated. The detection limit from the blank controls was 60 cells/well (n=6).

    References (3)

    Publishing research using ab112119? Please let us know so that we can cite the reference in this datasheet.

    ab112119 has been referenced in 3 publications.

    • Hernandez N  et al. Inherited IFNAR1 deficiency in otherwise healthy patients with adverse reaction to measles and yellow fever live vaccines. J Exp Med 216:2057-2070 (2019). PubMed: 31270247
    • Moens L  et al. A novel kindred with inherited STAT2 deficiency and severe viral illness. J Allergy Clin Immunol 139:1995-1997.e9 (2017). PubMed: 28087227
    • Saayman SM  et al. Potent and Targeted Activation of Latent HIV-1 Using the CRISPR/dCas9 Activator Complex. Mol Ther 24:488-98 (2016). PubMed: 26581162

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