FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab271217 in the following tested applications.
|ELISA||Use a concentration of 0.25 - 1 µg/ml.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa.|
FunctionHormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
Tissue specificityExpressed only in fatty tissues.
Sequence similaritiesBelongs to the resistin/FIZZ family.
- Information by UniProt
- Adipose tissue specific secretory factor antibody
- Adipose tissue-specific secretory factor antibody
- ADSF antibody
Western blot analysis of varying levels of Recombinant mouse Resistin protein using ab271217 at 0.2 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant Resistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Mouse Resistin was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271217. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Resistin protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271217 has not yet been referenced specifically in any publications.