Product nameAnti-Resistin antibody [EPR23388-272]
See all Resistin primary antibodies
DescriptionRabbit monoclonal [EPR23388-272] to Resistin
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: IHC-Fr or IHC-P
Species reactivityReacts with: Rat
Recombinant fragment within Rat Resistin aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q8K4J7
- WB: Rat white fat tissue lysate. IP: Rat white fat tissue lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab272730 in the following tested applications.
|WB||1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).|
FunctionHormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
Tissue specificityExpressed only in fatty tissues.
Sequence similaritiesBelongs to the resistin/FIZZ family.
- Information by UniProt
- Adipose tissue specific secretory factor antibody
- Adipose tissue-specific secretory factor antibody
- ADSF antibody
All lanes : Anti-Resistin antibody [EPR23388-272] (ab272730) at 1/1000 dilution
Lane 1 : Rat white fat tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat lung tissue lysate
Lysates/proteins at 40 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what have been described in the literature (PMID: 11201732 ).
Negative control: Rat brain, Rat lung (PMID: 11201732 ).
Exposure time: 37 seconds.
Resistin was immunoprecipitated from 0.35 mg Rat white fat tissue lysate 10ug with ab272730 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272730 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat white fat tissue lysate 10ug
Lane 2: ab272730 IP in Rat white fat tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272730 in Rat white fat tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272730 has not yet been referenced specifically in any publications.