Product nameAnti-Resistin antibody [EPR23388-272] - BSA and Azide free
See all Resistin primary antibodies
DescriptionRabbit monoclonal [EPR23388-272] to Resistin - BSA and Azide free
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: IHC-Fr or IHC-P
Species reactivityReacts with: Rat
Recombinant fragment within Rat Resistin aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q8K4J7
- WB: Rat white fat tissue lysate. IP: Rat white fat tissue lysate.
ab273094 is the carrier-free version of ab272730. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab273094 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab273094 in the following tested applications.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).|
FunctionHormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
Tissue specificityExpressed only in fatty tissues.
Sequence similaritiesBelongs to the resistin/FIZZ family.
- Information by UniProt
- Adipose tissue specific secretory factor antibody
- Adipose tissue-specific secretory factor antibody
- ADSF antibody
Resistin was immunoprecipitated from 0.35 mg Rat white fat tissue lysate 10ug with ab272730 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272730 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat white fat tissue lysate 10ug
Lane 2: ab272730 IP in Rat white fat tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272730).
ab273094 has not yet been referenced specifically in any publications.