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Protocols and troubleshooting
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p65, also known as RelA, is one of the five components that form transcription factor NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells). The other members are RelB, c-Rel and precursor proteins NF-κB1 (p50) and NF-κB2 (p52).
B cells are responsible for producing and releasing antibodies to specific antigens, and hence, are an essential component of the humoral immune response.
An overview of NF-κB signaling covering the canonical and non-canonical pathways, and NF-κB family members.
Find out more about IL-1 receptor I, its ligands IL-1α, IL-1β and IL-1RA, the coreceptor IL-1RAcP and how they activate the NF-κB, JNK, and p38 pathways.
Learn about single cell western blotting, a new approach to study protein expression on a cell-by-cell basis. Our products support this new technique
Read about the history of cell cycle research, the different phases of the cell cycle and the involvement of Cyclins and Cyclin-dependent kinases (Cdks) and download your own copy of the brand new cell cycle pathway card.
Learn about your options when you need a cell proliferation or cell cycle assay.
For cell viability, cytotoxicity, proliferation, cell cycle, or apoptosis assays, use this guide to understand your options
Review dyes used for labeling subcellular organelles, live cell tracking, and live cell labeling.
Discover our protocol describing the procedure of fluorescence activated cell sorting of live cell populations. Including tips to keep cells viable.