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Discover the procedure for separating nuclear, membrane and cytoplasmic cell fractions using centrifugation methods.
Find out the procedure for isolation of soluble mitochondrial proteins from mitochondrial suspension.
Solve your western blot problems with these troubleshooting tips, covering common causes of no signal, high background, multiple bands and more.
Discover our protocols and troubleshooting tips on how to get the best from our antibodies. Includes protocols for a range of applications and research areas.
Discover various sample preparations, including lysis buffers, lysate from cell culture, lysate from tissues and determination of protein concentration.
Whether you want nuclear, membrane, ER, or other organelle markers, we have the antibodies you need.
On-demand webinar providing useful hints and tips on identifying protein interactions through immunoprecipitation (IP) and overcoming biochemical problems that can be associated with this technique.
Discover our protocol for the isolation of mitochondria from cells for western blotting. Includes buffer recipes and a detailed procedure.
A decrease in mitochondrial transmembrane potential & release of cytochrome C are indicative of the final stages of apoptosis. Use our kits to monitor these events.