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Secondary antibody selection guide

A complete guide to secondary antibody selection, helping you choose the most appropriate secondary antibody for your application. Discover more information on antibody host species, isotype, pre-adsorption, and F(ab) fragments.

Isotype control guide

Discover more with the appropriate isotype control to validate your experiment. Isotype controls are certified for analysis in flow cytometry, immunohistochemistry, and other applications.

Pre-adsorbed secondary antibodies

Learn why use of pre-adsorbed secondary antibodies can minimize non-specific binding and high background staining

Protein A, G, L or jacalin?

Choosing the optimal affinity resin is key to affinity purification of antibodies, immunoprecipitation and ChIP. Commonly used immobilized proteins vary in their ability to bind antibodies of different species and subclass. Read our detailed selection guide.

Antibody purification and concentration kits

Abcam has over 10 purification and concentration kits. Our kits are suitable for purifying antibodies from crude samples, and for the concentration of antibodies and protein from a variety sample types.

Secondary antibodies for IHC

Choosing the right secondary antibody for immunohistochemistry (IHC) is essential when using indirect detection. Find out which factors to consider.

PEG RabMAb products for pharmacokinetics

Polyethylene Glycol (PEG) rabbit monoclonal antibodies and RabMAb products for pharmacokinetics studies, including absorption, distribution, metabolism, excretion and toxicity. Perfect for use in ELISA, WB and IHC.

Knockout (KO) validation: confirming antibody specificity

To ensure you have highly specific antibodies, we use KO validation via CRISPR/Cas9 genome-editing to give you the reliable results your research demands.

Secondaries you can trust

Our scientists have developed a range of well-defined secondary antibodies with extensive validation data in key applications.

Secondary antibodies for STED

We now have a range of secondary antibodies conjugated to ATTO fluorophores specifically engineered for STED microscopy.

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