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A complete guide to secondary antibody selection, helping you choose the most appropriate secondary antibody for your application. Discover more information on antibody host species, isotype, pre-adsorption, and F(ab) fragments.
Discover more with the appropriate isotype control to validate your experiment. Isotype controls are certified for analysis in flow cytometry, immunohistochemistry, and other applications.
Learn why use of pre-adsorbed secondary antibodies can minimize non-specific binding and high background staining
Choosing the optimal affinity resin is key to affinity purification of antibodies, immunoprecipitation and ChIP. Commonly used immobilized proteins vary in their ability to bind antibodies of different species and subclass. Read our detailed selection guide.
Abcam has over 10 purification and concentration kits. Our kits are suitable for purifying antibodies from crude samples, and for the concentration of antibodies and protein from a variety sample types.
Choosing the right secondary antibody for immunohistochemistry (IHC) is essential when using indirect detection. Find out which factors to consider.
Polyethylene Glycol (PEG) rabbit monoclonal antibodies and RabMAb products for pharmacokinetics studies, including absorption, distribution, metabolism, excretion and toxicity. Perfect for use in ELISA, WB and IHC.
To ensure you have highly specific antibodies, we use KO validation via CRISPR/Cas9 genome-editing to give you the reliable results your research demands.
Our scientists have developed a range of well-defined secondary antibodies with extensive validation data in key applications.
We now have a range of secondary antibodies conjugated to ATTO fluorophores specifically engineered for STED microscopy.