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Visit protocols and troubleshooting or check them out using the Abcam app for iPhone
Protocols and troubleshooting
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Discover more with the appropriate isotype control to validate your experiment. Isotype controls are certified for analysis in flow cytometry, immunohistochemistry, and other applications.
A complete guide to secondary antibody selection, helping you choose the most appropriate secondary antibody for your application. Discover more information on antibody host species, isotype, pre-adsorption, and F(ab) fragments.
Choosing the optimal affinity resin is key to affinity purification of antibodies, immunoprecipitation and ChIP. Commonly used immobilized proteins vary in their ability to bind antibodies of different species and subclass. Read our detailed selection guide.
Comprehensive guide to Human CD antigens.
General immunoprecipitation procedure including a list of reagents and a table to help you choose the correct protein beads.
Read about the structural components that make up an antibody, including heavy and light chains, F(ab) and Fc regions, and antibody isotypes.
Choosing the right secondary antibody matters. Learn about the many factors that may affect the specificity, sensitivity and consistency of secondary antibodies.
B cells are responsible for producing and releasing antibodies to specific antigens, and hence, are an essential component of the humoral immune response.
Hints and tips for experimental design and sample preparation in flow cytometry.