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Protocols and troubleshooting
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In-Cell ELISAs have several advantages over western blotting. They are easy to analyze in replicate, and there is no need for different gel concentration. Discover how in-cell ELISAs can benefit your research.
In this on-demand introductory webinar to In-Cell ELISA, our resident expert Dr John Constable explains the benefits of In-Cell technology, key reagents and optimizing techniques.
Discover our In-Cell ELISA (ICE) detailed protocol. Measure protein levels or post-translational modifications in cultured adherent cells.
There are several types of antibody validation techniques such as ELISA, siRNA, knockout cell lines, and others. Learn the pros and cons of each validation technique.
Create your own ELISA with sensitive and consistent monoclonal matched antibody pairs. Kits are available in 2 or 10 x 96-well plate sizes.
Check out our T cell activation interactive pathway and browse for antibodies, proteins and ELISA kits against targets such as CD3, CD28, CD45 and ZAP70.
Rapidly label your primary antibody or protein with fluorophores, enzymes or gold without affecting epitope recognition. With no downstream separation or clean-up steps you can quickly produce a directly labeled antibody & benefit from direct immunoassays. Learn more
In this on-demand webinar, our resident ELISA expert Dr Jeremy Kasanov gives comprehensive troubleshooting tips on how to develop your own ELISA.
Our guide to help you choose the correct secondary antibody conjugate for your scientific application (e.g. ELISA, western blotting, immunofluorescence, immunohistochemistry, flow cytometry and immunohistochemisstry).
A complete guide to secondary antibody selection, helping you choose the most appropriate secondary antibody for your application. Discover more information on antibody host species, isotype, pre-adsorption, and F(ab) fragments.