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Protocols and troubleshooting
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Histones pack and order the DNA into nucleosomes, the building blocks of chromatin. Each nucleosome contains two subunits each of histones, known as the core histones. They are characterized by a large number of post-translational modifications. Learn more.
Biotin forms large complexes with avidin or streptavidin for signal amplification. Learn about the different methods that use biotin-conjugated antibodies.
Watch this on-demand webinar to hear Jordan Plieskatt of the Bethony Lab at GWU discuss his labs' use of multiplexing technologies for multiple miRNA detections from crude matrices in a large number of samples as required for cancer screening.
Find out how to troubleshoot common ChIP problems such as high background in non-specific antibody controls, low signal, low resolution with high background across large regions and more.
Western blot protocols
Discover the process of the transfer of proteins in this section of our detailed guide to western blotting, including the visualization of proteins in gels.
Watch this on-demand applications of ChIP webinar to learn about epigenetics, ChIP and histone modifications.
In this on-demand introductory webinar to western blotting, our resident expert gives advice on sample preparation through to detection, as well as offering a few troubleshooting tips.
Dr. Khosrow Adeli presents the first webinar in a series of three focusing on metabolic mechanisms. Part one is titled Metabolic syndrome: Mechanisms, pathophysiology and laboratory assessment.