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Visit protocols and troubleshooting or check them out using the Abcam app for iPhone
Protocols and troubleshooting
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We offer a validated range of loading control antibodies. 19 targets are available in multiple pack sizes and several HRP conjugates. Use our table to find the right loading control for your western blots.
Found in all eukaryotic cells, actin is a protein essential to maintain cell structure. In humans, three main actin groups have been identified - alpha, beta. Actin is used as a loading control for western blotting to normalize protein loading. Read full article.
A review of our knockout validated antibody
Discover our detailed electrophoresis protocol, including the preparation of PAGE gels and loading controls.
Looking to optimize your western blot experiment? Discover our recommended controls for western blot.
Exlpore targets to the research process of actin polymerization
In this on-demand introductory webinar to western blotting, our resident expert gives advice on sample preparation through to detection, as well as offering a few troubleshooting tips.
Our western blot protocol is a quick guide to help with your experiment, and includes a list of solutions and reagents.
Discover various sample preparations, including lysis buffers, lysate from cell culture, lysate from tissues and determination of protein concentration.
To ensure you have highly specific antibodies, we use KO validation via CRISPR/Cas9 genome-editing to give you the reliable results your research demands.