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Calcium plays a critial role in various neuronal processes, including neurotransmittter release, membrane excitability and apoptosis. The guide explores how one ubiquitous intracellular messenger regulate so many diverse processes.
Protocols and guides on calcium signaling in neurons, cardiac myocytes, apoptosis and cancer.
The role calcium plays in apoptosis, focusing on the endoplasmic reticulum and mitochondria.
A detailed protocol that uses Fluo-3 AM in combination with flow cytometry to monitor T cell receptor-triggered calcium changes over time.
A detailed protocol on the measurement of free cytosolic calcium concentration in single CHO-K1 cells.
Fura-2 AM calcium imaging protocol for fibroblasts, PC12, embryonic neurons and more.
A detailed protocol with tips and tricks on how to image calcium using Fura-2 AM in motoro neurons from ventral spinal cord.
A calcium phosphate transfection protocol optimized for transfection of neonatal rat cardiac myocytes.
Find out about the roles of calcium signaling in cell excitability, exocytosis, motility, apoptosis, and transcription.
Find out more about fluorescent dyes and indicators for visualizing and detecting intracellular changes in calcium ions using fluorescence microscopy.