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Interest in RNA-protein interactions is booming as we begin to appreciate the role of RNA in epigenetics. RNA immunoprecipitation (RIP) allows mapping RNA–protein interactions in vivo by immunoprecipitating an RNA binding protein together with its associated RNA.
Non-coding RNAs play a big part in epigenetic regulation of gene expression. Studies have shown 90% of the eukaryotic genome is transcribed, of which only 1–2% of these transcripts encode for proteins. Read more about the importance of ncRNAs.
Discover our protocols and troubleshooting tips on how to get the best from our antibodies. Includes protocols for a range of applications and research areas.
Learn different applications and techniques for determining the presence and distribution of RNA modifications in mRNA, tRNA and more.
Get the best results from your RNA modification antibodies with these tips for control experiments
UV cross-linking and immunoprecipitation (CLIP) protocol. Includes reagents, protocols, and troubleshooting tips.
Find out the procedure for cross-linking chromatin immunoprecipitation (X-ChIP) with this detailed guide
On-demand webinar providing useful hints and tips on identifying protein interactions through immunoprecipitation (IP) and overcoming biochemical problems that can be associated with this technique.
Find out the procedure for RNA isolation, DNase treatment and reverse transcription in our detailed protocol.
Read our detailed troubleshooting tips for immunoprecipitation (IP) for problems including high background and a high amount of antibody eluting.