Overview

  • Product name
    Anti-Respiratory Syncytial Virus antibody [2F7]
    See all Respiratory Syncytial Virus primary antibodies
  • Description
    Mouse monoclonal [2F7] to Respiratory Syncytial Virus
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-Fr, ICC/IF, ELISAmore details
  • Species reactivity
    Respiratory Syncytial Virus (RSV) strains (Paramyxovirus 1, 2 & 3). No cross-reactivity with other respiratory viruses.
  • Immunogen

    Recombinant FP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    2F7
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 48 , 70 kDa.
IHC-Fr 1/100 - 1/500.
ICC/IF 1/100 - 1/500.

Immunocytology on infected cells.

 

 

ELISA 1/2000 - 1/1000.

Target

  • Relevance
    Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children. RSV infection produces a variety of signs and symptoms involving different areas of the respiratory tract, from the nose to the lungs. RSV is a negative sense, enveloped RNA virus. The virion is variable in shape and size with average diameter of between 120 and 300 nm. The 63 kD RSV fusion protein of the RSS 2 strain (subtype A) directs fusion of viral and cellular membranes, results in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia or multi nucleated giant cells.
  • Cellular localization
    Virion. Host cytoplasm
  • Alternative names
    • RS virus antibody
    • RSV antibody

Images

  • Immunofluorescent staining of Respiratory Syncytial Virus F protein Bound to HeLa cells using ab43812. Respiratory Syncytial Virus F protein was visualized in green and Respiratory Syncytial N protein in red.

References

This product has been referenced in:
  • Mehedi M  et al. Multicolor Stimulated Emission Depletion (STED) Microscopy to Generate High-resolution Images of Respiratory Syncytial Virus Particles and Infected Cells. Bio Protoc 7:N/A (2017). Read more (PubMed: 29057295) »
  • Liang B  et al. Improved Prefusion Stability, Optimized Codon Usage, and Augmented Virion Packaging Enhance the Immunogenicity of Respiratory Syncytial Virus Fusion Protein in a Vectored-Vaccine Candidate. J Virol 91:N/A (2017). Read more (PubMed: 28539444) »
See all 11 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Vielen Dank für Ihre Rückmeldung.

Das war mein Fehler: Wir erhalten diesen Antikörper vom Labor im lyophilizierten Zustand (Menge von 100ug), und versetzen ihn mit 200ul Wasser, aber Verkaufen 100ul Größen. Das bedeutet, dass die Endkonzentration 0.5 mg/ml beträgt. Den wichtigen Löseschritt habe ich leider völlig Überlesen!

Es tut mir leid, dass ich Sie verwirrt habe.



Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

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Answer


Nach einigem Hin und Her haben wir das Datenblatt auf den neusten Stand gebracht: Es handelt sich um einen aufgereinigten Antikörper mit einer Konzentration von 1 mg/ml in folgenden Puffer: Tris 0,1M, glycine 0,1M, sucrose 2%

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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