Anti-Respiratory Syncytial Virus antibody (ab20745)
- Datasheet
- Specific References (16)
- Protocols
Overview
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Product nameAnti-Respiratory Syncytial Virus antibody
See all Respiratory Syncytial Virus primary antibodies -
DescriptionGoat polyclonal to Respiratory Syncytial Virus
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Host speciesGoat
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Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ELISA, Conjugationmore details
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Species reactivityReacts with: Respiratory syncytial virus
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Immunogen
Tissue, cells or virus corresponding to Respiratory Syncytial Virus. Viral lysate of Human RSV isolate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 0.01M PBS, pH 7.2 -
Concentration information loading... -
PurityIgG fraction
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Purification notesSodium sulphate precipitation and DEAE chromatography.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab20745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent dilution. PubMed: 20647383 | |
| IHC-P | Use at an assay dependent dilution. | |
| WB | Use at an assay dependent dilution. | |
| ELISA | Use at an assay dependent dilution. | |
| Conjugation | Use at an assay dependent dilution. |
Target
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RelevanceRespiratory syncytial virus (RSV) is a major cause of respiratory illness in young children. RSV infection produces a variety of signs and symptoms involving different areas of the respiratory tract, from the nose to the lungs. RSV is a negative sense, enveloped RNA virus. The virion is variable in shape and size with average diameter of between 120 and 300 nm. The 63 kD RSV fusion protein of the RSS 2 strain (subtype A) directs fusion of viral and cellular membranes, results in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia or multi nucleated giant cells.
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Cellular localizationVirion. Host cytoplasm
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Database links
- Entrez Gene: 2829063 Respiratory syncytial virus
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Alternative names
- RS virus antibody
- RSV antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Respiratory Syncytial Virus antibody (ab20745)Image from Munday DC et al., Mol Cell Proteomics. 2010 Nov;9(11):2438-59. Epub 2010 Jul 20. Fig 1.; doi: 10.1074/mcp.M110.001859; November 1, 2010 Molecular & Cellular Proteomics, 9, 2438-2459.Immunofluorescence analysis of A549 cells infected with RSV, staining Respiratory Syncytial Virus with ab20745.
Cells were fixed with formalin before staining with primary antibody, followed by ab6881 - Donkey polyclonal Secondary Antibody to Goat IgG - H&L (FITC).
Protocols
References
This product has been referenced in:
- Machado D et al. RSV Infection in Human Macrophages Promotes CXCL10/IP-10 Expression during Bacterial Co-Infection. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 29215596) »
- Munday DC et al. Interactome analysis of the human respiratory syncytial virus RNA polymerase complex identifies protein chaperones as important cofactors that promote L-protein stability and RNA synthesis. J Virol 89:917-30 (2015). WB ; Human . Read more (PubMed: 25355874) »
Customer reviews and Q&As
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