Overview

  • Product name

    Anti-Respiratory Syncytial Virus Fusion (F) Glycoprotein antibody [RSV5A6]
    See all Respiratory Syncytial Virus Fusion (F) Glycoprotein primary antibodies
  • Description

    Mouse monoclonal [RSV5A6] to Respiratory Syncytial Virus Fusion (F) Glycoprotein
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, WB, ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Human Respiratory Syncytial Virus strain A2 infected HeLa cells

  • General notes

    Fusion partner: PS-NS/1-Ag4


    ab94968 is useful for the identification and location of expression of the fusion glycoprotein of Human Respiratory Syncytial Virus of both sub-groups A and B.

Properties

Applications

Our Abpromise guarantee covers the use of ab94968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Fix with Acetone.

Target

  • Relevance

    Respiratory Syncytial Virus (RSV) Fusion (F) Glycoprotein is a Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (protein F) interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between host cell and virion membranes. Notably, RSV fusion protein is able to interact directly with heparan sulfate and therefore actively participates in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.
  • Cellular localization

    Virion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass membrane protein.
  • Alternative names

    • F antibody
    • Fusion glycoprotein F0 antibody
    • Protein F antibody
    • RSV Fusion (F) Glycoprotein antibody

References

This product has been referenced in:

  • Li M  et al. Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis. J Virol 92:N/A (2018). Read more (PubMed: 29386287) »
  • Vanover D  et al. RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane. Nat Commun 8:667 (2017). Read more (PubMed: 28939853) »
See all 3 Publications for this product

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