Key features and details
- Mouse monoclonal [RSV5A6] to Respiratory Syncytial Virus Fusion (F) Glycoprotein
- Suitable for: ELISA, WB, ICC/IF, IHC-P, IHC-Fr
- Isotype: IgG2a
Product nameAnti-Respiratory Syncytial Virus Fusion (F) Glycoprotein antibody [RSV5A6]
See all Respiratory Syncytial Virus Fusion (F) Glycoprotein primary antibodies
DescriptionMouse monoclonal [RSV5A6] to Respiratory Syncytial Virus Fusion (F) Glycoprotein
Tested applicationsSuitable for: ELISA, WB, ICC/IF, IHC-P, IHC-Frmore details
Species reactivityReacts with: Other species
Human Respiratory Syncytial Virus strain A2 infected HeLa cells
General notesFusion partner: PS-NS/1-Ag4
ab94968 is useful for the identification and location of expression of the fusion glycoprotein of Human Respiratory Syncytial Virus of both sub-groups A and B.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Primary antibody notesab94968 is useful for the identification and location of expression of the fusion glycoprotein of Human Respiratory Syncytial Virus of both sub-groups A and B.
Our Abpromise guarantee covers the use of ab94968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. Fix with Acetone.|
RelevanceRespiratory Syncytial Virus (RSV) Fusion (F) Glycoprotein is a Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (protein F) interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between host cell and virion membranes. Notably, RSV fusion protein is able to interact directly with heparan sulfate and therefore actively participates in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.
Cellular localizationVirion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass membrane protein.
- F antibody
- Fusion glycoprotein F0 antibody
- Protein F antibody
- RSV Fusion (F) Glycoprotein antibody
ab94968 has been referenced in 5 publications.
- Touzelet O et al. The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins. Mol Cell Proteomics 19:793-807 (2020). PubMed: 32075873
- Li J et al. Long Noncoding RNA NRAV Promotes Respiratory Syncytial Virus Replication by Targeting the MicroRNA miR-509-3p/Rab5c Axis To Regulate Vesicle Transportation. J Virol 94:N/A (2020). PubMed: 32102886
- Li M et al. Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis. J Virol 92:N/A (2018). PubMed: 29386287
- Vanover D et al. RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane. Nat Commun 8:667 (2017). PubMed: 28939853
- West WH et al. Biological activity, binding site and affinity of monoclonal antibodies to the fusion protein of respiratory syncytial virus. J Gen Virol 75 ( Pt 10):2813-9 (1994). PubMed: 7931172