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    respiratory-syncytial-virus-fusion-protein-antibody-rsv3216-b016-ab24011.pdf

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Microbiology Organism Virus RNA Virus single stranded RNA negative strand virus Resp. Syncytial Virus
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Anti-Respiratory Syncytial Virus fusion protein antibody [RSV3216 (B016)] (ab24011)

  • Datasheet
Reviews (1) Submit a question References (4)

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Key features and details

  • Mouse monoclonal [RSV3216 (B016)] to Respiratory Syncytial Virus fusion protein
  • Suitable for: Flow Cyt, WB, IP, ICC/IF, Electron Microscopy, Sandwich ELISA
  • Reacts with: Respiratory syncytial virus
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-Respiratory Syncytial Virus fusion protein antibody [RSV3216 (B016)]
  • Description

    Mouse monoclonal [RSV3216 (B016)] to Respiratory Syncytial Virus fusion protein
  • Host species

    Mouse
  • Specificity

    Recognises a Respiratory Syncytial Virus fusion protein (46kD and 22kD s-s linked glycoprotein).
  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ICC/IF, Electron Microscopy, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Respiratory syncytial virus
  • Immunogen

    Tissue, cells or virus corresponding to Respiratory Syncytial Virus fusion protein. Bovine RSV strains: 127, SNK and 9007. Human RSV strains: Long, Randall, 8/60, and A/2.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.50
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    RSV3216 (B016)
  • Myeloma

    NS1
  • Isotype

    IgG2b
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • single stranded RNA negative strand virus
    • Resp. Syncytial Virus

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • sELISA pair antibody

    • Anti-Respiratory Syncytial Virus Nucleoprotein antibody [B023] (ab22501)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab24011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (1)
Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Electron Microscopy
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [B023] to Respiratory Syncytial Virus Nucleoprotein (ab22501). Use ab34992 as capture antibody
Notes
Flow Cyt
Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Electron Microscopy
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [B023] to Respiratory Syncytial Virus Nucleoprotein (ab22501). Use ab34992 as capture antibody

Target

  • Relevance

    Respiratory Syncytial Virus fusion protein directs fusion of viral and cellular membranes, resulting in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia.
  • Cellular localization

    Cell Membrane
  • Database links

    • Entrez Gene: 1494475 Respiratory syncytial virus
    • Alternative names

      • Fusion glycoprotein F0 antibody
      • Fusion glycoprotein F1 antibody
      • Fusion glycoprotein F2 antibody
      • Fusion protein (F) antibody
      • RSV fusion protein antibody
      see all

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (4)

    Publishing research using ab24011? Please let us know so that we can cite the reference in this datasheet.

    ab24011 has been referenced in 4 publications.

    • Du X  et al. Respiratory syncytial virus infection-induced mucus secretion by down-regulation of miR-34b/c-5p expression in airway epithelial cells. J Cell Mol Med 24:12694-12705 (2020). PubMed: 32939938
    • Hu T  et al. TLR4 and nucleolin influence cell injury, apoptosis and inflammatory factor expression in respiratory syncytial virus-infected N2a neuronal cells. J Cell Biochem 120:16206-16218 (2019). PubMed: 31081244
    • Yuan X  et al. Respiratory syncytial virus prolifically infects N2a neuronal cells, leading to TLR4 and nucleolin protein modulations and RSV F protein co-localization with TLR4 and nucleolin. J Biomed Sci 25:13 (2018). Mouse . PubMed: 29427996
    • Jans J  et al. Actin- and clathrin-dependent mechanisms regulate interferon gamma release after stimulation of human immune cells with respiratory syncytial virus. Virol J 13:52 (2016). PubMed: 27004689

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Flow Cytometry abreview for Anti-Respiratory Syncytial Virus fusion protein antibody [RSV3216 (B016)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    Fix/Perm
    Permeabilization
    Yes - Perm/Wash (BD Biosciences)
    Sample
    Human Cell (CD14+ PBMC infected with RSV)
    Specification
    CD14+ PBMC infected with RSV
    Gating Strategy
    PBMCs were gated on CD14
    Preparation
    Cell harvesting/tissue preparation method: Ficoll isolated PBMCs infected with RSV with moi of 1
    Sample buffer: CytoFix/CytoPerm (BD Biosciences)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 21 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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