Anti-Respiratory Syncytial Virus G Glycoprotein antibody [RSV133] (ab94966)

Submit a review Q&A (2)References (5)

Key features and details

  • Mouse monoclonal [RSV133] to Respiratory Syncytial Virus G Glycoprotein
  • Suitable for: ELISA, WB, ICC/IF, IHC-Fr
  • Isotype: IgG1

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Anti-VSV-G tag antibody [EPR12997] (ab183497)
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  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Respiratory Syncytial Virus G Glycoprotein antibody [RSV133]

  • Description

    Mouse monoclonal [RSV133] to Respiratory Syncytial Virus G Glycoprotein

  • Host species

    Mouse

  • Tested applications

    Suitable for: ELISA, WB, ICC/IF, IHC-Frmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Human Respiratory Syncytial Virus strain A2 infected HeLa cells

  • General notes

    Fusion partner: PS-NS/1-Ag4


    ab94966 is useful for the identification and location of expression of the G glycoprotein of Human Respiratory Syncytial Virus (HRSV) of both sub-groups A and B . This antibody confers passive protection against HRSV of both subgroups in an animal model of hRSV infection.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Primary antibody notes

    ab94966 is useful for the identification and location of expression of the G glycoprotein of Human Respiratory Syncytial Virus (HRSV) of both sub-groups A and B . This antibody confers passive protection against HRSV of both subgroups in an animal model of hRSV infection.

  • Clonality

    Monoclonal

  • Clone number

    RSV133

  • Isotype

    IgG1

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab94966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration. Fix with Acetone.
Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration. Fix with Acetone.

Target

  • Relevance

    Respiratory Syncytial Virus (RSV) G Glycoprotein attaches the virion to the host cell membrane by interacting with heparan sulfate, initiating the infection. It interacts with host CX3CR1, the receptor for the CX3C chemokine fractalkine, to modulate the immune response and facilitate infection. Unlike the other paramyxovirus attachment proteins, it lacks both neuraminidase and hemagglutinating activities.

  • Cellular localization

    Virion membrane. Host cell surface

  • Alternative names

    • Attachment glycoprotein G antibody
    • G antibody
    • Major surface glycoprotein G antibody
    • Membrane bound glycoprotein antibody
    • mG antibody
    • RSV G Glycoprotein antibody
    see all

References (5)

Publishing research using ab94966? Please let us know so that we can cite the reference in this datasheet.

ab94966 has been referenced in 5 publications.

  • Chathuranga K  et al. Anti-Respiratory Syncytial Virus Activity of Plantago asiatica and Clerodendrum trichotomum Extracts In Vitro and In Vivo. Viruses 11:N/A (2019). PubMed: 31277257
  • Tiwari PM  et al. Engineered mRNA-expressed antibodies prevent respiratory syncytial virus infection. Nat Commun 9:3999 (2018). PubMed: 30275522
  • Vanover D  et al. RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane. Nat Commun 8:667 (2017). PubMed: 28939853
  • Shi H  et al. Baicalin from Scutellaria baicalensis blocks respiratory syncytial virus (RSV) infection and reduces inflammatory cell infiltration and lung injury in mice. Sci Rep 6:35851 (2016). PubMed: 27767097
  • Le Nouën C  et al. Attenuation of human respiratory syncytial virus by genome-scale codon-pair deoptimization. Proc Natl Acad Sci U S A 111:13169-74 (2014). PubMed: 25157129

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

To whom it concerns,

I am interested in receiving more information about you MAb 94966, an anti-RSV G protein MAb, with a view to purchasing it. You indicate that the MAb confers passive protection against both RSV A and B subtypes. However, you do not provide information about the epitope within G that is recognised. Could you provide this information for me please?

Thanks in advance.

Best regards,

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Answer

Thank you for your inquiry.

I am very sorry for the delay in my answer.

I was trying to find the source of the statement: "This antibody confers passive protection against HRSV of both subgroups in an animal model of hRSV infection."

Unfortunately, this statement was a personal communication of the scientist that developed this monoclonal antibody and I cannot provide a publication for citation.

I can confirm that the epitope of ab94966 was not further mapped.

I am sorry I could not be of more help on this occasion and hope this information is nevertheless helpful.

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Question

Inquiry: To whom it concerns, You were contacted on the 11th of June about receiving more information regarding your MAb 94966, an anti-RSV G protein MAb, with a view to purchasing it: "You indicate that the MAb confers passive protection against both RSV A and B subtypes. However, you do not provide information about the epitope within G that is recognised. Could you provide this information for me please?" Since he hasn't had any answer from you, except that someone was working on it, I would like to follow-up on the request as it is becoming urgent for us to order that Ab, or not. Thanks in advance. Best regards,

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Answer

Thank you for contacting us.
We used Human RSV strain A2 infected HeLa cells to raise this antibody. The exact epitope sequence has not been determined so I am sorry we do not have, any extra information, other than what is already on the datasheet. This antibody is suitable for detection of virus Glycoprotein in ELISA, WB, ICC/IF, IHC-Fr and is fully covered under Abpromise guarantee.
I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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