Key features and details
- Rabbit polyclonal to REST / NRSF
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-REST / NRSF antibody
See all REST / NRSF primary antibodies
DescriptionRabbit polyclonal to REST / NRSF
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human gastric and Mouse brain tissues
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab202962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 122 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods.
FunctionTranscriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
Tissue specificityUbiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
Sequence similaritiesContains 9 C2H2-type zinc fingers.
- Information by UniProt
- Neural Restrictive Silencer Factor antibody
- Neural-restrictive silencer factor antibody
- Neuron Restrictive Silencer Factor antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded Mouse brain tissue labeling REST / NRSF with ab202962 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Anti-REST / NRSF antibody (ab202962) at 1/300 dilution + Raji cell lysates
Predicted band size: 122 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human gastric tissue staining ab202962 (1/200).
ab202962 has been referenced in 2 publications.
- Bray JK et al. Loss of RE-1 silencing transcription factor accelerates exocrine damage from pancreatic injury. Cell Death Dis 11:138 (2020). PubMed: 32080178
- Jin H et al. Local Delivery of miR-21 Stabilizes Fibrous Caps in Vulnerable Atherosclerotic Lesions. Mol Ther 26:1040-1055 (2018). PubMed: 29503197