Overview

  • Product name
  • Description
    Rabbit polyclonal to REST / NRSF
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Mouse REST/ NRSF.

    Read Abcam's proprietary immunogen policy (Peptide available as ab17042.)

  • Positive control
    • This antibody gave a positive signal in HeLa and HEK293 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab21635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 21715642Fixed for 10 min in cold methanol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
    • Tissue specificity
      Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
    • Sequence similarities
      Contains 9 C2H2-type zinc fingers.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Neural Restrictive Silencer Factor antibody
      • Neural-restrictive silencer factor antibody
      • Neuron Restrictive Silencer Factor antibody
      • NRSF antibody
      • RE1 silencing transcription factor antibody
      • RE1-silencing transcription factor antibody
      • Repressor binding to the X2 box antibody
      • rest antibody
      • REST protein antibody
      • REST_HUMAN antibody
      • X2 box repressor antibody
      • XBR antibody
      see all

    Images

    • All lanes : Anti-REST / NRSF antibody (ab21635) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Mouse REST / NRSF peptide (ab17042) at 1 µg/ml
      Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Mouse REST / NRSF peptide (ab17042) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 121 kDa
      Observed band size: 121 kDa
      Additional bands at: 53 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 10 minutes

    References

    This product has been referenced in:
    • Liu Q  et al. Decreased hyperpolarization-activated cyclic nucleotide-gated channels are involved in bladder dysfunction associated with spinal cord injury. Int J Mol Med 41:2609-2618 (2018). Read more (PubMed: 29436607) »
    • Yu Y  et al. NRSF/REST levels are decreased in cholangiocellular carcinoma but not hepatocellular carcinoma compared with normal liver tissues: A tissue microarray study. Oncol Lett 15:6592-6598 (2018). Read more (PubMed: 29725406) »
    See all 14 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Application
    Western blot
    Sample
    Rat Tissue lysate - nuclear (Hippocampus, liver and MCF7 whole cell homogenates)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    80 µg
    Specification
    Hippocampus, liver and MCF7 whole cell homogenates
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Octavio Mercado-Gomez

    Verified customer

    Submitted May 12 2016

    Application
    Western blot
    Loading amount
    90 µg
    Gel Running Conditions
    Reduced Denaturing (4-18% gel)
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
    Specification
    Mouse embryonic stem cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Mar 17 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    Yes - 0.3% TRITON-X
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jul 14 2014

    Answer

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality. Reviewing this case, I would like to offer some suggestions to help optimise the results from ab21635. I would also appreciate if you can confirm some further details: 1. I am sorry to confirm this antibody has not previously been tested in Oct embedded sections. Have you tried in fresh frozen sections, not embedded, which are tested and guaranteed? I can recommend to try this alternative if possible. 2. I can suggest there is no need to fix in methanol, as the tissue is already fixed n 4% PFA. If you want to be sure, I can recommend to fix in 4% PFA for a further 10 minutes before proceeding with staining. 2. 0.5% is quite a high concentration of Triton. This is a strong detergent which may disrupt proteins. Try 0.1% for 10 minutes only. This will help permeabilize the cells and nucleus. 3. Could you confirm if the current vial of secondary antibody is working well with other primary antibodies. In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

    Read More

    Answer

    Thank you for contacting us. I have forwarded your data to our lab for analysis. It would be very helpful if you could please provide some additional details about your protocol: 1.) How were the tissues fixed and processed (paraffin or frozen, PFA fixation, etc)? We have only validated this antibody for use on PFA-fixed frozen sections. 2.) Did you perform any antigen retrieval, permeabilization, or other pretreatments? 3.) How were the sections blocked? 4.) What were the primary and secondary antibody dilutions and incubation times? 5.) What secondary antibody was used? Did the no primary antibody control come out clean? 6.) Could you please provide your original order or lot number? I will be happy to assist you further if you could please provide this additional information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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