Key features and details
- Rabbit polyclonal to REST / NRSF
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-REST / NRSF antibody
See all REST / NRSF primary antibodies
DescriptionRabbit polyclonal to REST / NRSF
Tested applicationsSuitable for: IHC-P, IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 800 and 850 of human REST/ NRSF
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70300 was affinity purified using an epitope specific to REST / NRSF immobilized on solid support.
Our Abpromise guarantee covers the use of ab70300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionTranscriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
Tissue specificityUbiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
Sequence similaritiesContains 9 C2H2-type zinc fingers.
- Information by UniProt
- Neural Restrictive Silencer Factor antibody
- Neural-restrictive silencer factor antibody
- Neuron Restrictive Silencer Factor antibody
ab70300 has not yet been referenced specifically in any publications.