Overview

  • Product name

    Anti-REST / NRSF antibody [CL0381]
    See all REST / NRSF primary antibodies
  • Description

    Mouse monoclonal [CL0381] to REST / NRSF
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment (proprietary-tag) corresponding to Human REST/ NRSF aa 864-991.
    Sequence:

    SPPLPKENLREEASGDQKLLNTGEGNKEAPLQKVGAEEADESLPGLAANI NESTHISSSGQNLNTPEGETLNGKHQTDSIVCEMKMDTDQNTRENLTGIN STVEEPVSPMLPPSAVEEREAVSKTALA


    Database link: Q13127

  • Positive control

    • Human tonsil, fallopian tube, testis, kidney, liver and cortex tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab211537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
  • Tissue specificity

    Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
  • Sequence similarities

    Contains 9 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Neural Restrictive Silencer Factor antibody
    • Neural-restrictive silencer factor antibody
    • Neuron Restrictive Silencer Factor antibody
    • NRSF antibody
    • RE1 silencing transcription factor antibody
    • RE1-silencing transcription factor antibody
    • Repressor binding to the X2 box antibody
    • rest antibody
    • REST protein antibody
    • REST_HUMAN antibody
    • X2 box repressor antibody
    • XBR antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling REST / NRSF with ab211537 at 1/50 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cortex tissue labeling REST / NRSF with ab211537 at 1/50 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling REST / NRSF with ab211537 at 1/50 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling REST / NRSF with ab211537 at 1/50 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fallopian tube tissue labeling REST / NRSF with ab211537 at 1/50 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling REST / NRSF with ab211537 at 1/50 dilutio.

References

ab211537 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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