For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    reticulocalbin-3-antibody-ab204178.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Signaling Pathway Calcium Signaling Calcium Binding Proteins
Share by email

Anti-Reticulocalbin 3 antibody (ab204178)

  • Datasheet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Floating vibratome sections) - Anti-Reticulocalbin 3 antibody (ab204178)
  • Western blot - Anti-Reticulocalbin 3 antibody (ab204178)
  • Immunocytochemistry/ Immunofluorescence - Anti-Reticulocalbin 3 antibody (ab204178)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (ab204178)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (ab204178)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (ab204178)

Key features and details

  • Rabbit polyclonal to Reticulocalbin 3
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Reticulocalbin 3 protein (ab123203)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
Product image
Anti-Psoriasin antibody [EPR23482-38] (ab275026)

View more associated products

Overview

  • Product name

    Anti-Reticulocalbin 3 antibody
  • Description

    Rabbit polyclonal to Reticulocalbin 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human Reticulocalbin 3 aa 150-300.
    Database link: Q96D15

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human cerebral cortex, upper gastrointestinal tract, testis, and skin tissues; ICC: U-2 OS cells; WB: AN3-CA cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Reticulocalbin 3 protein (ab123203)
  • Related Products

    • Recombinant Human Reticulocalbin 3 protein (ab123203)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab204178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
IHC-P
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 37 kDa.
Notes
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
IHC-P
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 37 kDa.

Target

  • Sequence similarities

    Belongs to the CREC family.
    Contains 6 EF-hand domains.
  • Cellular localization

    Endoplasmic reticulum lumen.
  • Target information above from: UniProt accession Q96D15 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 57333 Human
    • Entrez Gene: 52377 Mouse
    • Entrez Gene: 494125 Rat
    • SwissProt: Q96D15 Human
    • SwissProt: Q8BH97 Mouse
    • Unigene: 567550 Human
    • Alternative names

      • EF-hand calcium-binding protein RLP49 antibody
      • RCN3 antibody
      • RCN3_HUMAN antibody
      • reticulocalbin 3, EF-hand calcium binding domain antibody
      • Reticulocalbin-3 antibody
      • RLP49 antibody
      • UNQ239/PRO272 antibody
      see all

    Images

    • Immunohistochemistry (Floating vibratome sections) - Anti-Reticulocalbin 3 antibody (ab204178)
      Immunohistochemistry (Floating vibratome sections) - Anti-Reticulocalbin 3 antibody (ab204178)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human upper gastrointestinal tract tissue labelling Reticulocalbin 3 with ab204178 at 1/2500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-Reticulocalbin 3 antibody (ab204178)
      Western blot - Anti-Reticulocalbin 3 antibody (ab204178)
      Anti-Reticulocalbin 3 antibody (ab204178) at 0.4 µg/ml + AN3-CA (human endometrium adenocarcinoma cell line) cell lysate

      Predicted band size: 37 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Reticulocalbin 3 antibody (ab204178)
      Immunocytochemistry/ Immunofluorescence - Anti-Reticulocalbin 3 antibody (ab204178)

      Immunofluorescent analysis of PFA-fixed,Triton X-100 permeabilized U-2 OS cells labeling Reticulocalbin 3 with ab204178 at 4 µg/ml (green).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (ab204178)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (ab204178)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Reticulocalbin 3 with ab204178 at 1/2500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (ab204178)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (ab204178)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling Reticulocalbin 3 with ab204178 at 1/2500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (ab204178)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (ab204178)

      Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Reticulocalbin 3 with ab204178 at 1/2500 dilution.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab204178? Please let us know so that we can cite the reference in this datasheet.

    ab204178 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab204178.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.