Anti-Reticulocalbin 3 antibody (ab204178)
Key features and details
- Rabbit polyclonal to Reticulocalbin 3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Reticulocalbin 3 antibody -
Description
Rabbit polyclonal to Reticulocalbin 3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human Reticulocalbin 3 aa 150-300.
Database link: Q96D15 -
Positive control
- IHC-P: Human cerebral cortex, upper gastrointestinal tract, testis, and skin tissues; ICC: U-2 OS cells; WB: AN3-CA cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 37 kDa.
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| Notes |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 37 kDa. |
Target
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Sequence similarities
Belongs to the CREC family.
Contains 6 EF-hand domains. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 57333 Human
- Entrez Gene: 52377 Mouse
- Entrez Gene: 494125 Rat
- SwissProt: Q96D15 Human
- SwissProt: Q8BH97 Mouse
- Unigene: 567550 Human
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Alternative names
- EF-hand calcium-binding protein RLP49 antibody
- RCN3 antibody
- RCN3_HUMAN antibody
see all
Images
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Immunohistochemistry (Floating vibratome sections) - Anti-Reticulocalbin 3 antibody (ab204178)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human upper gastrointestinal tract tissue labelling Reticulocalbin 3 with ab204178 at 1/2500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-Reticulocalbin 3 antibody (ab204178)Anti-Reticulocalbin 3 antibody (ab204178) at 0.4 µg/ml + AN3-CA (human endometrium adenocarcinoma cell line) cell lysate
Predicted band size: 37 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Reticulocalbin 3 antibody (ab204178)Immunofluorescent analysis of PFA-fixed,Triton X-100 permeabilized U-2 OS cells labeling Reticulocalbin 3 with ab204178 at 4 µg/ml (green).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (ab204178)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Reticulocalbin 3 with ab204178 at 1/2500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (ab204178)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling Reticulocalbin 3 with ab204178 at 1/2500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (ab204178)Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Reticulocalbin 3 with ab204178 at 1/2500 dilution.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab204178 has not yet been referenced specifically in any publications.
