Anti-Reticulon 1A antibody [MON162] (ab9274)
- Datasheet
- References (7)
- Protocols
Overview
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Product nameAnti-Reticulon 1A antibody [MON162]
See all Reticulon 1A primary antibodies -
DescriptionMouse monoclonal [MON162] to Reticulon 1A
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Host speciesMouse
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SpecificityThis antibody exclusively recognizes the 135 kD Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues
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Tested applicationsSuitable for: IHC-P, ICC/IF, WB, IHC-Fr, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Hamster, Human, Pig
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Immunogen
Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).
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EpitopeThe epitope of this antibody is located between amino acid residues 338-422 of Reticulon-1A.
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General notes
A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein G purified
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Primary antibody notesA central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.
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ClonalityMonoclonal
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Clone numberMON162
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Myelomaunknown
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab9274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| WB | 1/100 - 1/1000. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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RelevanceReticulon 1A may be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. It is expressed in neural and neuroendocrine tissues and cell cultures derived therefrom. Expression of isoform RTN1-C is strongly correlated with neuronal differentiation. Different isoforms have been described as a result of alternative splicing.
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Cellular localizationEndoplasmic reticulum
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Database links
- Entrez Gene: 6252 Human
- Entrez Gene: 104001 Mouse
- Entrez Gene: 116644 Rat
- Omim: 600865 Human
- SwissProt: Q16799 Human
- SwissProt: Q8K0T0 Mouse
- SwissProt: Q64548 Rat
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Alternative names
- Neuroendocrine specific protein antibody
- NSP antibody
- Reticulon1 antibody
- RTN1 antibody
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Reticulon 1A antibody [MON162] (ab9274)](https://www.abcam.com/ps/datasheet/images/9/ab9274/Reticulon-1A-Primary-antibodies-ab9274-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Reticulon 1A antibody [MON162] (ab9274)Immunostaining of NCI-H82 variant small cell lung cancer -
![Immunohistochemistry (Frozen sections) - Anti-Reticulon 1A antibody [MON162] (ab9274)](https://www.abcam.com/ps/datasheet/images/9/ab9274/Reticulon-1A-Primary-antibodies-ab9274-2.jpg)
Immunohistochemistry (Frozen sections) - Anti-Reticulon 1A antibody [MON162] (ab9274)Immunohistochemistry on frozen section of swine brain
Datasheets and documents
Protocols
References
This product has been referenced in:
- Grumati P et al. Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy. Elife 6:N/A (2017). WB . Read more (PubMed: 28617241) »
- Xu W et al. Normal fibroblasts induce E-cadherin loss and increase lymph node metastasis in gastric cancer. PLoS One 9:e97306 (2014). Read more (PubMed: 24845259) »
