Overview

  • Product name
    Anti-Reticulon 1A antibody [MON162]
    See all Reticulon 1A primary antibodies
  • Description
    Mouse monoclonal [MON162] to Reticulon 1A
  • Host species
    Mouse
  • Specificity
    This antibody exclusively recognizes the 135 kD Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human, Pig
  • Immunogen

    Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).

  • Epitope
    The epitope of this antibody is located between amino acid residues 338-422 of Reticulon-1A.
  • General notes


    A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.
  • Clonality
    Monoclonal
  • Clone number
    MON162
  • Myeloma
    unknown
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB 1/100 - 1/1000.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    Reticulon 1A may be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. It is expressed in neural and neuroendocrine tissues and cell cultures derived therefrom. Expression of isoform RTN1-C is strongly correlated with neuronal differentiation. Different isoforms have been described as a result of alternative splicing.
  • Cellular localization
    Endoplasmic reticulum
  • Database links
    • Alternative names
      • Neuroendocrine specific protein antibody
      • NSP antibody
      • Reticulon1 antibody
      • RTN1 antibody

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Reticulon 1A antibody [MON162] (ab9274)
      Immunocytochemistry/ Immunofluorescence - Anti-Reticulon 1A antibody [MON162] (ab9274)
      Immunostaining of NCI-H82 variant small cell lung cancer
    • Immunohistochemistry (Frozen sections) - Anti-Reticulon 1A antibody [MON162] (ab9274)
      Immunohistochemistry (Frozen sections) - Anti-Reticulon 1A antibody [MON162] (ab9274)
      Immunohistochemistry on frozen section of swine brain

    References

    This product has been referenced in:
    • Grumati P  et al. Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy. Elife 6:N/A (2017). WB . Read more (PubMed: 28617241) »
    • Xu W  et al. Normal fibroblasts induce E-cadherin loss and increase lymph node metastasis in gastric cancer. PLoS One 9:e97306 (2014). Read more (PubMed: 24845259) »

    See all 7 Publications for this product

    Publishing research using ab9274? Please let us know so that we can cite the reference in this datasheet.

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