Anti-Reticulon 1A antibody [MON162] (ab9274)
![Immunocytochemistry/ Immunofluorescence - Anti-Reticulon 1A antibody [MON162] (ab9274)](https://www.abcam.com/ps/products/9/ab9274/Images/ab9274-16882-anti-reticulon-1a-antibody-mon162-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Reticulon 1A antibody [MON162] (ab9274)")
Key features and details
- Mouse monoclonal [MON162] to Reticulon 1A
- Suitable for: ICC/IF, IHC-Fr
- Reacts with: Human, Pig
- Isotype: IgG1
Overview
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Product name
Anti-Reticulon 1A antibody [MON162]
See all Reticulon 1A primary antibodies -
Description
Mouse monoclonal [MON162] to Reticulon 1A -
Host species
Mouse -
Specificity
This antibody exclusively recognizes the 135 kD Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-Fr Pig
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Immunogen
Fusion protein corresponding to Reticulon 1A aa 6-776. Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).
Database link: Q16799 -
Epitope
The epitope of this antibody is located between amino acid residues 338-422 of Reticulon-1A. -
General notes
A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution. -
Clonality
Monoclonal -
Clone number
MON162 -
Myeloma
unknown -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
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| IHC-Fr |
Pig
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
Target
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Relevance
Reticulon 1A may be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. It is expressed in neural and neuroendocrine tissues and cell cultures derived therefrom. Expression of isoform RTN1-C is strongly correlated with neuronal differentiation. Different isoforms have been described as a result of alternative splicing. -
Cellular localization
Endoplasmic reticulum -
Database links
- Entrez Gene: 6252 Human
- Omim: 600865 Human
- SwissProt: Q16799 Human
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Alternative names
- Neuroendocrine specific protein antibody
- NSP antibody
- Reticulon1 antibody
- RTN1 antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Reticulon 1A antibody [MON162] (ab9274)
Immunostaining of NCI-H82 (a human cell line) variant small cell lung cancer
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![Immunohistochemistry (Frozen sections) - Anti-Reticulon 1A antibody [MON162] (ab9274)](https://www.abcam.com/ps/products/9/ab9274/Images/ab9274-16881-anti-reticulon-1a-antibody-mon162-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Reticulon 1A antibody [MON162] (ab9274)Immunohistochemistry on frozen section of swine brain
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab9274 has been referenced in 9 publications.
- Qin T et al. Tumor necrosis factor superfamily 15 promotes lymphatic metastasis via upregulation of vascular endothelial growth factor-C in a mouse model of lung cancer. Cancer Sci 109:2469-2478 (2018). PubMed: 29890027
- McGonigle TA et al. Reticulon-1 and Reduced Migration toward Chemoattractants by Macrophages Differentiated from the Bone Marrow of Ultraviolet-Irradiated and Ultraviolet-Chimeric Mice. J Immunol 200:260-270 (2018). PubMed: 29167231
- Grumati P et al. Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy. Elife 6:N/A (2017). WB . PubMed: 28617241
- Xu W et al. Normal fibroblasts induce E-cadherin loss and increase lymph node metastasis in gastric cancer. PLoS One 9:e97306 (2014). PubMed: 24845259
- Hens J et al. Neuronal differentiation is accompanied by NSP-C expression. Cell Tissue Res 292:229-37 (1998). PubMed: 9560466
- Senden NH et al. A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers. J Pathol 182:13-21 (1997). PubMed: 9227337
- van de Velde HJ et al. NSP-encoded reticulons are neuroendocrine markers of a novel category in human lung cancer diagnosis. Cancer Res 54:4769-76 (1994). PubMed: 8062278
- van de Velde HJ et al. NSP-encoded reticulons, neuroendocrine proteins of a novel gene family associated with membranes of the endoplasmic reticulum. J Cell Sci 107 ( Pt 9):2403-16 (1994). PubMed: 7844160
- van de Velde HJ et al. Molecular analysis of expression in rat brain of NSP-A, a novel neuroendocrine-specific protein of the endoplasmic reticulum. Brain Res Mol Brain Res 23:81-92 (1994). PubMed: 7518032