Anti-Retinal protein 4 antibody (ab169652)
Key features and details
- Mouse polyclonal to Retinal protein 4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Retinal protein 4 antibody
See all Retinal protein 4 primary antibodies -
Description
Mouse polyclonal to Retinal protein 4 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog -
Immunogen
Recombinant full length protein within Human Retinal protein 4 aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Lysate from 293T cells transfected with Retinal protein 4; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Binds myristoylated GNAT1 and is required for G-protein localization and trafficking in sensory neurons. Binds myristoylated NPHP3; however, in contrast to UNC119B, does not seem to play a major role in ciliary membrane localization of NPHP3. Does not bind all myristoylated proteins. Probably plays a role in trafficking proteins in photoreceptor cells. -
Tissue specificity
Retinal-specific. Localized in photoreceptor synapses in the outer plexiform layer of the retina. -
Involvement in disease
Note=Defects in UNC119 may be a cause of cone-rod dystrophy. A mutation was found in a 57-year-old woman with late-onset cone-rod dystrophy: from 40 year old, the patient suffered from poor night vision, defective color vision and light-sensitivity. At 57 year old, she displayed reduced visual acuity, myopa, macular atrophy and pericentral ring scotomas. The disease was caused by a heterozygous mutation causing premature termination and truncated UNC119 protein with dominant-negative effect. -
Sequence similarities
Belongs to the PDE6D/unc-119 family. -
Domain
Adopts an immunoglobulin-like beta-sandwich fold forming a hydrophobic cavity that capture N-terminally myristoylated target peptides (PubMed:21642972). Phe residues within the hydrophobic beta sandwich are required for myristate binding (PubMed:22085962). -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 538501 Cow
- Entrez Gene: 491169 Dog
- Entrez Gene: 9094 Human
- Entrez Gene: 22248 Mouse
- Entrez Gene: 29402 Rat
- Omim: 604011 Human
- SwissProt: Q3SYR2 Cow
- SwissProt: O19177 Dog
see all -
Alternative names
- Cone-rod dystrophy, included antibody
- hRG4 antibody
- Human retinal gene 4 antibody
see all
Images
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All lanes : Anti-Retinal protein 4 antibody (ab169652) at 1 µg/ml
Lane 1 : Retinal protein 4-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 27 kDa -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling Retinal protein 4 using ab169652 at 10 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab169652 has not yet been referenced specifically in any publications.