Overview

  • Product name

    Anti-Retinal protein 4 antibody
    See all Retinal protein 4 primary antibodies
  • Description

    Rabbit polyclonal to Retinal protein 4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment within Human Retinal protein 4 (internal sequence). The exact sequence is proprietary.
    Database link: Q13432

  • Positive control

    • WB: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.

Target

  • Function

    Myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Binds myristoylated GNAT1 and is required for G-protein localization and trafficking in sensory neurons. Binds myristoylated NPHP3; however, in contrast to UNC119B, does not seem to play a major role in ciliary membrane localization of NPHP3. Does not bind all myristoylated proteins. Probably plays a role in trafficking proteins in photoreceptor cells.
  • Tissue specificity

    Retinal-specific. Localized in photoreceptor synapses in the outer plexiform layer of the retina.
  • Involvement in disease

    Note=Defects in UNC119 may be a cause of cone-rod dystrophy. A mutation was found in a 57-year-old woman with late-onset cone-rod dystrophy: from 40 year old, the patient suffered from poor night vision, defective color vision and light-sensitivity. At 57 year old, she displayed reduced visual acuity, myopa, macular atrophy and pericentral ring scotomas. The disease was caused by a heterozygous mutation causing premature termination and truncated UNC119 protein with dominant-negative effect.
  • Sequence similarities

    Belongs to the PDE6D/unc-119 family.
  • Domain

    Adopts an immunoglobulin-like beta-sandwich fold forming a hydrophobic cavity that capture N-terminally myristoylated target peptides (PubMed:21642972). Phe residues within the hydrophobic beta sandwich are required for myristate binding (PubMed:22085962).
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cone-rod dystrophy, included antibody
    • hRG4 antibody
    • Human retinal gene 4 antibody
    • MRG4 antibody
    • POC7 antibody
    • POC7 centriolar protein homolog A antibody
    • POC7A antibody
    • Protein unc 119 homolog A antibody
    • Protein unc-119 homolog A antibody
    • Retinal protein 4 antibody
    • RG4 antibody
    • RRG4 antibody
    • Rtg4 antibody
    • U119A_HUMAN antibody
    • unc 119 homolog (C. elegans) antibody
    • Unc 119 protein homolog antibody
    • unc119 (C.elegans) homolog antibody
    • UNC119 antibody
    • Unc119h antibody
    • Uncl19 antibody
    see all

Images

  • Anti-Retinal protein 4 antibody (ab228952) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 27 kDa



    12% SDS-PAGE

References

ab228952 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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