Key features and details
- Rabbit polyclonal to Retinal protein 4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Retinal protein 4 antibody
See all Retinal protein 4 primary antibodies
DescriptionRabbit polyclonal to Retinal protein 4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
- IHC-P: Human retina tissue. WB: Retinal protein 4 overexpressed HEK-293T whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254865 in the following tested applications.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 51 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMyristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Binds myristoylated GNAT1 and is required for G-protein localization and trafficking in sensory neurons. Binds myristoylated NPHP3; however, in contrast to UNC119B, does not seem to play a major role in ciliary membrane localization of NPHP3. Does not bind all myristoylated proteins. Probably plays a role in trafficking proteins in photoreceptor cells.
Tissue specificityRetinal-specific. Localized in photoreceptor synapses in the outer plexiform layer of the retina.
Involvement in diseaseNote=Defects in UNC119 may be a cause of cone-rod dystrophy. A mutation was found in a 57-year-old woman with late-onset cone-rod dystrophy: from 40 year old, the patient suffered from poor night vision, defective color vision and light-sensitivity. At 57 year old, she displayed reduced visual acuity, myopa, macular atrophy and pericentral ring scotomas. The disease was caused by a heterozygous mutation causing premature termination and truncated UNC119 protein with dominant-negative effect.
Sequence similaritiesBelongs to the PDE6D/unc-119 family.
DomainAdopts an immunoglobulin-like beta-sandwich fold forming a hydrophobic cavity that capture N-terminally myristoylated target peptides (PubMed:21642972). Phe residues within the hydrophobic beta sandwich are required for myristate binding (PubMed:22085962).
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- Cone-rod dystrophy, included antibody
- hRG4 antibody
- Human retinal gene 4 antibody
Paraffin-embedded human retina tissue stained for Retinal protein 4 using ab254865 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-Retinal protein 4 antibody (ab254865) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T cell lysate
Lane 2 : Retinal protein 4 overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Predicted band size: 51 kDa
ab254865 has not yet been referenced specifically in any publications.