Anti-Retinal S antigen antibody (ab3435)
Key features and details
- Rabbit polyclonal to Retinal S antigen
- Suitable for: WB
- Reacts with: Cow
- Isotype: IgG
Overview
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Product name
Anti-Retinal S antigen antibody
See all Retinal S antigen primary antibodies -
Description
Rabbit polyclonal to Retinal S antigen -
Host species
Rabbit -
Specificity
Detects recombinant bovine visual Arrestin. -
Tested Applications & Species
See all applications and species dataApplication Species WB Cow
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Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to, and interpreted by, the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. Arrestins are cytosolic proteins that are involved in G protein-coupled receptor (GPCR) desensitization. Arrestin binding to activated GPCRs is phosphorylation dependent and, once bound, uncouple the GPCR from the associated heterotrimeric G proteins. There are currently 4 known mammalian isoforms, beta-Arrestin 1 (Arrestin 2), beta-Arrestin 2 (Arrestin 3), visual Arrestin (Arrestin 1), and cone arrestin. The beta- isoforms are ubiquitously expressed and are known to interact with acetylcholine and adrenergic receptors. Visual and cone Arrestins are found to interact directly with transducin. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Cow
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| All applications |
Rat
Dog
Pig
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 2 µg/ml. |
Target
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Function
Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated-phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase. -
Tissue specificity
Retina and pineal gland. -
Involvement in disease
Defects in SAG are a cause of congenital stationary night blindness Oguchi type 1 (CSNBO1) [MIM:258100]; also known as Oguchi disease. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision. CSNBO is an autosomal recessive form associated with fundus discoloration and abnormally slow dark adaptation. -
Sequence similarities
Belongs to the arrestin family. - Information by UniProt
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Database links
- Entrez Gene: 280922 Cow
- Entrez Gene: 403906 Dog
- Entrez Gene: 397151 Pig
- Entrez Gene: 25539 Rat
- SwissProt: P08168 Cow
- SwissProt: P79260 Pig
- SwissProt: P15887 Rat
- Unigene: 9856 Rat
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Alternative names
- 48 kDa protein antibody
- Arrestin 1 antibody
- Arrestin antibody
see all
Images
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Western blot - Anti-Retinal S antigen antibody (ab3435)Western blot on recombinant bovine Retinal S antigen using ab3435.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab3435 has been referenced in 1 publication.
- Olivares AM et al. The nuclear hormone receptor gene Nr2c1 (Tr2) is a critical regulator of early retina cell patterning. Dev Biol 429:343-355 (2017). IHC-P ; Mouse . PubMed: 28551284