Key features and details
- Rabbit polyclonal to Retinoblastoma binding protein 6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Retinoblastoma binding protein 6 antibody
See all Retinoblastoma binding protein 6 primary antibodies
DescriptionRabbit polyclonal to Retinoblastoma binding protein 6
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- WB: Mouse brain tissue lysate. IHC-P: Human cervical cancer and testis tissue. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 202 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionE3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth.
Tissue specificityHighly expressed in the placenta and testis. Expressed at lower levels in the brain, heart, kidney, liver and lung. Overexpressed in esophageal cancer.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 CCHC-type zinc finger.
Contains 1 DWNN domain.
Contains 1 RING-type zinc finger.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated by NEK6.
Cellular localizationNucleus > nucleolus. Chromosome. Cytoplasm > cytoskeleton > centrosome. Colocalizes with mitotic chromosomes. Co-localizes with NEK6 in the centrosome.
- Information by UniProt
- DKFZp686P0638 antibody
- DKFZp761B2423 antibody
- E3 ubiquitin-protein ligase RBBP6 antibody
Anti-Retinoblastoma binding protein 6 antibody (ab237514) at 1/500 dilution + Mouse brain tissue lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 202 kDa
Paraffin-embedded human cervical cancer tissue stained for Retinoblastoma binding protein 6 using ab237514 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for Retinoblastoma binding protein 6 using ab237514 at 1/100 dilution in immunohistochemical analysis.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Retinoblastoma binding protein 6 (green) using ab237514 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237514 has not yet been referenced specifically in any publications.