Key features and details
- Rabbit polyclonal to retinoblastoma like 2 - C-terminal
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-retinoblastoma like 2 antibody - C-terminal
DescriptionRabbit polyclonal to retinoblastoma like 2 - C-terminal
Tested applicationsSuitable for: IHC-P, IP, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human retinoblastoma like 2 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). (NP_005602.3)
Database link: Q08999
- Human brain cerebellum tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesab189647 has been delipidated and defibrinated.
Our Abpromise guarantee covers the use of ab189647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500 - 1/2000. Predicted molecular weight: 128 kDa.|
RelevanceRetinoblastoma like 2 is directly involved in heterochromatin formation and probably acts as a transcription repressor by recruiting chromatin modifying enzymes to promoters. It is a potent inhibitor of E2F mediated transactivation and associates preferentially with E2F5. It also binds to cyclins A and E. It binds to and may be involved in the transforming capacity of the adenovirus E1A protein. It may also act as a tumor suppressor.
- 130 kDa retinoblastoma associated protein antibody
- FLJ26459 antibody
- H sapiens p130 mRNA for 130K protein antibody
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human brain cerebellum sections labeling retinoblastoma like 2 using ab189647 at a 1/500 dilution. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
ab189647 has not yet been referenced specifically in any publications.