Key features and details
- Goat polyclonal to Retinoic Acid Receptor alpha
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Retinoic Acid Receptor alpha antibody
See all Retinoic Acid Receptor alpha primary antibodies
DescriptionGoat polyclonal to Retinoic Acid Receptor alpha
SpecificityThis antibody is expected to recognize all three reported [human] isoforms (NP_000955.1; NP_001019980.1; NP_001028775.1).
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Chicken, Hamster, Cow, Dog
- WB: Human breast and Human breast cancer tissue lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Retinoic & Retinoid
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab28767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).Can be blocked with Retinoic Acid Receptor alpha peptide (ab4910).
We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature.
FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing MLL5. Mediates retinoic acid-induced granulopoiesis.
Involvement in diseaseNote=Chromosomal aberrations involving RARA are commonly found in acute promyelocytic leukemia. Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM. The PML-RARA oncoprotein requires both the PML ring structure and coiled-coil domain for both interaction with UBE2I, nuclear microspeckle location and sumoylation. In addition, the coiled-coil domain functions in blocking RA-mediated transactivation and cell differentiation.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsPhosphorylated on serine and threonine residues. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity). Phosphorylation by AKT1 is required for the repressor activity but has no effect on DNA binding, protein stability nor subcellular localization. Phosporylated by PKA in vitro. This phosphorylation on Ser-219 and Ser-369 is critical for ligand binding, nuclear localization and transcriptional activity in response to FSH signaling.
Sumoylated by SUMO2, mainly on Lys-399 which is also required for SENP6 binding. On all-trans retinoic acid (ATRA) binding, a confromational change may occur that allows sumoylation on two additional site, Lys-166 and Lys-171. Probably desumoylated by SENP6. Sumoylation levels determine nuclear localization and regulate ATRA-mediated transcriptional activity.
Trimethylation enhances heterodimerization with RXRA and positively modulates the transcriptional activation.
Cellular localizationNucleus. Cytoplasm. Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation.
- Information by UniProt
- NR1B1 antibody
- Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein antibody
- Nuclear receptor subfamily 1 group B member 1 antibody
All lanes : Anti-Retinoic Acid Receptor alpha antibody (ab28767) at 1 µg/ml
Lane 1 : Human Breast tissue lysate
Lane 2 : Human Breast cancer tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
ab28767 has been referenced in 16 publications.
- Goncalves MB et al. Regulation of Myelination by Exosome Associated Retinoic Acid Release from NG2-Positive Cells. J Neurosci 39:3013-3027 (2019). PubMed: 30760627
- El Zein RM et al. Retinoic acid receptor a as a novel contributor to adrenal cortex structure and function through interactions with Wnt and Vegfa signalling. Sci Rep 9:14677 (2019). PubMed: 31605007
- Bertini Teixeira M et al. Fasciculation and elongation zeta-1 protein (FEZ1) interacts with the retinoic acid receptor and participates in transcriptional regulation of theHoxb4gene. FEBS Open Bio 8:4-14 (2018). PubMed: 29321952
- Chen J et al. Inhibition of cancer stem cell like cells by a synthetic retinoid. Nat Commun 9:1406 (2018). PubMed: 29643385
- Ashton A et al. Rhythmic Diurnal Synthesis and Signaling of Retinoic Acid in the Rat Pineal Gland and Its Action to Rapidly Downregulate ERK Phosphorylation. Mol Neurobiol 55:8219-8235 (2018). PubMed: 29520716