Anti-Retinoic Acid Receptor alpha antibody (ab28767)

Overview

  • Product name
    Anti-Retinoic Acid Receptor alpha antibody
    See all Retinoic Acid Receptor alpha primary antibodies
  • Description
    Goat polyclonal to Retinoic Acid Receptor alpha
  • Host species
    Goat
  • Specificity
    This antibody is expected to recognize all three reported [human] isoforms (NP_000955.1; NP_001019980.1; NP_001028775.1).
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Hamster, Cow, Dog
  • Immunogen

    Synthetic peptide:

    CSPSLSPSSH RSSPATQSP

    , corresponding to C terminal amino acids 441-459 of Retinoic Acid Receptor alpha

  • Positive control
    • WB: Human brain lysate. IHC-P: Human cerebellum tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab28767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

See Abreview.

WB Use a concentration of 0.03 - 0.3 µg/ml. Detects a band of approximately 48, 50 kDa (predicted molecular weight: 51 kDa).Can be blocked with Retinoic Acid Receptor alpha peptide (ab4910).
IHC-P Use a concentration of 4 - 6 µg/ml. Steamed antigen retrieval is recommended using citrate buffer pH 6.0.

Target

  • Function
    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing MLL5. Mediates retinoic acid-induced granulopoiesis.
  • Involvement in disease
    Note=Chromosomal aberrations involving RARA are commonly found in acute promyelocytic leukemia. Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM. The PML-RARA oncoprotein requires both the PML ring structure and coiled-coil domain for both interaction with UBE2I, nuclear microspeckle location and sumoylation. In addition, the coiled-coil domain functions in blocking RA-mediated transactivation and cell differentiation.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity). Phosphorylation by AKT1 is required for the repressor activity but has no effect on DNA binding, protein stability nor subcellular localization. Phosporylated by PKA in vitro. This phosphorylation on Ser-219 and Ser-369 is critical for ligand binding, nuclear localization and transcriptional activity in response to FSH signaling.
    Sumoylated by SUMO2, mainly on Lys-399 which is also required for SENP6 binding. On all-trans retinoic acid (ATRA) binding, a confromational change may occur that allows sumoylation on two additional site, Lys-166 and Lys-171. Probably desumoylated by SENP6. Sumoylation levels determine nuclear localization and regulate ATRA-mediated transcriptional activity.
    Trimethylation enhances heterodimerization with RXRA and positively modulates the transcriptional activation.
    Ubiquitinated.
  • Cellular localization
    Nucleus. Cytoplasm. Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • NR1B1 antibody
    • Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein antibody
    • Nuclear receptor subfamily 1 group B member 1 antibody
    • Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form antibody
    • RAR alpha antibody
    • RAR antibody
    • RAR-alpha antibody
    • rara antibody
    • RARA_HUMAN antibody
    • RARalpha antibody
    • RARalpha1 antibody
    • Retinoic acid nuclear receptor alpha variant 1 antibody
    • Retinoic acid nuclear receptor alpha variant 2 antibody
    • Retinoic acid receptor alpha antibody
    • Retinoic acid receptor alpha polypeptide antibody
    see all

Images

  • Anti-Retinoic Acid Receptor alpha antibody (ab28767) at 0.03 µg/ml + human brain lysate (30µg total protein per lane)

    Developed using the ECL technique.

    Predicted band size: 51 kDa
    Observed band size: 48,50 kDa (why is the actual band size different from the predicted?)

  • ab28767 at 4µg/ml staining Retinoic Acid Receptor alpha in human cerebellum tissue. Steamed antigen retrieval using citrate buffer pH6 was performed. AP staining. Image shows staining of Purkinje cytoplasm.

References

This product has been referenced in:
  • Bertini Teixeira M  et al. Fasciculation and elongation zeta-1 protein (FEZ1) interacts with the retinoic acid receptor and participates in transcriptional regulation of theHoxb4gene. FEBS Open Bio 8:4-14 (2018). Read more (PubMed: 29321952) »
  • Chen J  et al. Inhibition of cancer stem cell like cells by a synthetic retinoid. Nat Commun 9:1406 (2018). Read more (PubMed: 29643385) »

See all 11 Publications for this product

Customer reviews and Q&As

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (intestine)
Antigen retrieval step
None
Permeabilization
No
Specification
intestine
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 02 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO-K1 cells expressing Human Retinoic Acid Recept)
Specification
CHO-K1 cells expressing Human Retinoic Acid Recept
Fixative
Formaldehyde
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Mahesh Mathrubutham

Verified customer

Submitted Oct 24 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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