The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
Involvement in disease
Microphthalmia, syndromic, 12
Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
ab15515 staining Reinoic Acid Receptor beta at 1/100 dilution in human testis by Immunohistochemistry (FFPE-sections).
Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody (ab15515)This image is courtesy of an Abreview submitted by Dr Mahesh Mathrubutham
ab15515 at 1/100 staining hamster CHO-K1 cells expressing human retinoic acid receptor by ICC/IF. The cells were formaldehyde fixed and blocked before incubation with the antibody for 1 hour at 22°C. A FITC labelled goat anti-rabbit antibody was used as the secondary.
Childs AJ et al. Retinoic Acid signalling and the control of meiotic entry in the human fetal gonad. PLoS One6:e20249 (2011).
Read more (PubMed: 21674038) »
Fabricius EM et al. Model examination of chemoprevention with retinoids in squamous cell carcinomas of the head and neck region and suitable biomarkers for chemoprevention. Int J Oncol39:1083-97 (2011).
Read more (PubMed: 21785820) »