Overview

  • Product name
    Anti-Retinoic Acid Receptor beta antibody
    See all Retinoic Acid Receptor beta primary antibodies
  • Description
    Rabbit polyclonal to Retinoic Acid Receptor beta
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 351-390 of Human Retinoic Acid Receptor beta

  • Positive control
    • Human breast carcinoma tissue HepG2 cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab53161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 20655472
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
  • Involvement in disease
    Microphthalmia, syndromic, 12
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization
    Cytoplasm and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HAP antibody
    • HBV-activated protein antibody
    • NR1B2 antibody
    • Nuclear receptor subfamily 1 group B member 2 antibody
    • RAR B antibody
    • RAR beta antibody
    • RAR epsilon antibody
    • RAR-beta antibody
    • RAR-epsilon antibody
    • RARB antibody
    • RARB_HUMAN antibody
    • Retinoic acid receptor beta 2 antibody
    • Retinoic acid receptor beta 4 antibody
    • Retinoic acid receptor beta 5 antibody
    • Retinoic acid receptor beta antibody
    • Retinoic acid receptor beta polypeptide antibody
    • RRB2 antibody
    see all

Images

  • This image shows human breast carcinoma tissue stained with ab53161 at 1/50 dilution. The left hand image shows untreated tissue; the right hand image shows tissue treated with the immunising peptide.
  • All lanes : Anti-Retinoic Acid Receptor beta antibody (ab53161) at 1/500 dilution

    Lane 1 : HepG2 cell extract, untreated.
    Lane 2 : HepG2 cell extract treated with the immunising peptide.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

  • ICC/IF image of ab53161 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Chen J  et al. Inhibition of cancer stem cell like cells by a synthetic retinoid. Nat Commun 9:1406 (2018). Read more (PubMed: 29643385) »
  • Cortes E  et al. RAR-ß is downregulated in HCC & cirrhosis and its expression inhibits myosin-driven activation and durotaxis in hepatic stellate cells. Hepatology N/A:N/A (2018). Read more (PubMed: 30055117) »
See all 12 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (adult testis)
Specification
adult testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. 煜欽 林

Verified customer

Submitted Jan 18 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Marmoset (common) Tissue sections (marmoset adult testis section)
Specification
marmoset adult testis section
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. 煜欽 林

Verified customer

Submitted Jan 18 2013

Answer

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab53161 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 120 days of purchase), and if it appears that the antibody is at fault, a replacement/credit note/refund will be offered. I have looked through the protocols you used and have a few questions and suggestions that might help you resolve the problem. a) Multiple non-specific bands: What are the band sizes you obtained? The bands located above the expected molecular weight band could be multimers. Please try boiling your protein in SDS-PAGE for 10 minutes to ensure proper disruption of multimers. This will ensure the protein is in the correct conformation to run at the correct molecular weight and be detected by the antibody. Meanwhile, bands located below the expected molecular weight may indicate that your target protein has been digested. Please make sure that you incorporated sufficient protease inhibitors and have kept your samples on ice the whole time. In most cases, the cause of multiple bands is because the primary and/or secondary antibody concentration is too high and causes non-specific binding. The dilution that we have on the datasheet is a recommended starting dilution and customers are encouraged to determine the optimal concentration/dilution. Please try decreasing the primary (1:500 ~ 1:3000) antibody concentration. I would also suspect that the secondary antibody is binding non-specifically and causing the extra bands. Please run a no-primary control (without the primary antibody) as this will eliminate the possibility that your secondary antibody is the cause of the problem. b) Weak band at the correct location: Was the lysis buffer you used as strong as RIPA buffer? Please try RIPA buffer as it is the strongest and will ensure that you get all the protein of interest out. I would suggest increasing the amount of protein sample. At Abcam, we recommend using around 20-40ug per well and you may want to try increasing your sample to 40ug per well. Note: a high amount of protein sample may also cause multiple bands. So, please try the troubleshooting tips in section (a) before trying this section. I hope the above recommendations may already help you, if you still experience problems please do not hesitate to contact me with the answers to the above questions and an image of the blot if possible. I would also appreciate if you could also provide details of your order (purchase order number, shipping address/purchasing agent information) so that I can immediately arrange for a replacement or refund to you. Also, please advice on how you would to proceed with this enquiry.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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