Key features and details
- Mouse polyclonal to Retinoic Acid Receptor beta
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Retinoic Acid Receptor beta antibody
See all Retinoic Acid Receptor beta primary antibodies
DescriptionMouse polyclonal to Retinoic Acid Receptor beta
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length protein, corresponding to amino acids 1-448 of Human Retinoic Acid Receptor beta
- HeLa cells RARB transfected 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Retinoic & Retinoid
Our Abpromise guarantee covers the use of ab76778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
Involvement in diseaseMicrophthalmia, syndromic, 12
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Cellular localizationCytoplasm and Nucleus.
- Information by UniProt
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All lanes : Anti-Retinoic Acid Receptor beta antibody (ab76778) at 1/500 dilution
Lane 1 : Retinoic Acid Receptor beta transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
HeLa cells labelled with ab76778 at 10 µg/ml followed by fluorescein conjugated secondary antibody.
ab76778 has not yet been referenced specifically in any publications.