Product nameAnti-Retinoic Acid Receptor beta antibody [EPR2017]
See all Retinoic Acid Receptor beta primary antibodies
DescriptionRabbit monoclonal [EPR2017] to Retinoic Acid Receptor beta
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Retinoic Acid Receptor beta aa 400 to the C-terminus.
- Human kidney tissue; MCF7, MDA-MB-435, Daudi and U-87 MG cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.13 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Retinoic & Retinoid
Our Abpromise guarantee covers the use of ab124701 in the following tested applications.
|WB||1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
Involvement in diseaseMicrophthalmia, syndromic, 12
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Cellular localizationCytoplasm and Nucleus.
- Information by UniProt
- HAP antibody
- HBV-activated protein antibody
- NR1B2 antibody
All lanes : Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701) at 1/1000 dilution
Lane 1 : MCF7 cell lysates
Lane 2 : MDA-MB-435 cell lysates
Lane 3 : Daudi cell lysates
Lane 4 : U-87 MG cell lysates
Lysates/proteins at 10 µg per lane.
Lane 1 : Goat anti-Rabbit HRP at 1/20000 dilution
Lanes 2-4 : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 50 kDa
ab124701 at 1/100 dilution staining Retinoic Acid Receptor beta in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Wang W et al. High Expression of RARß Is a Favorable Factor in Colorectal Cancer. Dis Markers 2019:7138754 (2019). Read more (PubMed: 30944670) »
- Greussing R et al. Identification of microRNA-mRNA functional interactions in UVB-induced senescence of human diploid fibroblasts. BMC Genomics 14:224 (2013). WB . Read more (PubMed: 23557329) »