Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2017] to Retinoic Acid Receptor beta
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Product nameAnti-Retinoic Acid Receptor beta antibody [EPR2017]
See all Retinoic Acid Receptor beta primary antibodies
DescriptionRabbit monoclonal [EPR2017] to Retinoic Acid Receptor beta
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Retinoic Acid Receptor beta aa 400 to the C-terminus.
- WB: Daudi, MCF7, MDA-MB-435, and U-87 MG lysates. IHC-P: human bladder carcinoma and kidney tissues. ICC/IF: HCT 116 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.13 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Retinoic & Retinoid
Our Abpromise guarantee covers the use of ab124701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
Involvement in diseaseMicrophthalmia, syndromic, 12
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Cellular localizationCytoplasm and Nucleus.
- Information by UniProt
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Immunocytochemistry/ Immunofluorescence analysis of HCT 116 (Human colorectal carcinoma epithelial cell) cells labeling Retinoic Acid Receptor beta with Purified ab124701 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling Retinoic Acid Receptor beta with Purified ab124701 at 1:100 dilution (10.3 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
All lanes : Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701) at 1/2000 dilution (Purified)
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates
Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
All lanes : Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701) at 1/1000 dilution
Lane 1 : MCF7 cell lysates
Lane 2 : MDA-MB-435 cell lysates
Lane 3 : Daudi cell lysates
Lane 4 : U-87 MG cell lysates
Lysates/proteins at 10 µg per lane.
Lane 1 : Goat anti-Rabbit HRP at 1/20000 dilution
Lanes 2-4 : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 50 kDa
ab124701 at 1/100 dilution staining Retinoic Acid Receptor beta in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124701 has been referenced in 2 publications.
- Wang W et al. High Expression of RARß Is a Favorable Factor in Colorectal Cancer. Dis Markers 2019:7138754 (2019). PubMed: 30944670
- Greussing R et al. Identification of microRNA-mRNA functional interactions in UVB-induced senescence of human diploid fibroblasts. BMC Genomics 14:224 (2013). WB . PubMed: 23557329