Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)

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Overview

  • Product name
    Anti-Retinoic Acid Receptor gamma 1 antibody
  • Description
    Rabbit polyclonal to Retinoic Acid Receptor gamma 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide corresponding to Retinoic Acid Receptor gamma 1 aa 40-57 (N terminal).
    Sequence:

    EMLSPSFRGLGQPDLPKE


    (Peptide available as ab39773)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab5904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human Retinoic Acid Receptor gamma 1 peptide (ab39773).
IHC-P Use at an assay dependent concentration.
EMSA Use 1µl for 102 cells.

Use 1-2µl. ab5904 has been shown to efficiently super shift the human RAR gamma-1/retinoid X receptor (RXR)/RAR response element complex in gel super shift experiments using a number of different response elements.

Target

  • Function
    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • NR1B3 antibody
      • Nuclear receptor subfamily 1 group B member 3 antibody
      • RAR gamma antibody
      • RAR gamma isoform 1 antibody
      • RAR-gamma antibody
      • RARC antibody
      • Rarg antibody
      • RARG_HUMAN antibody
      • Retinoic acid receptor gamma antibody
      see all

    Images

    • Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
      Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
      All lanes : Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)

      Lane 1 : HEK293 cell lysate with neutralizing peptide
      Lane 2 : HEK293 cell lysate

      Predicted band size: 54 kDa

    References

    This product has been referenced in:
    • Joss-Moore L  et al. Intrauterine growth restriction transiently delays alveolar formation and disrupts retinoic acid receptor expression in the lung of female rat pups. Pediatr Res 73:612-20 (2013). Read more (PubMed: 23419538) »
    See 1 Publication for this product

    Publishing research using ab5904? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Inquiry: Hi Sir, please tell about the quantity of Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904) product in ug. as its not given there in the product information...! looking for a prompt reply as i have to do ChIP tomorrow. Thanks in advance.

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    Answer

    Thank you for your enquiry.

    I can confirm that ab5904 Retinoic Acid Receptor gamma 1 antibody is sold as whole antiserum.

    Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

    I can confirm that for whole antiserum, concentration of antibody is known to very between 1 - 10 mg/ml.


    I also would like to make you aware that ab5904 is not tested in CHIP and therefore is not covered by our guarantee for CHIP.
    We wouldlike to receive feedback on how this antibody performed in your CHIP experiment.


    I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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