Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Retinoic Acid Receptor gamma 1 antibody
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DescriptionRabbit polyclonal to Retinoic Acid Receptor gamma 1
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Host speciesRabbit
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Tested applicationsSuitable for: IP, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit
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Immunogen
Synthetic peptide corresponding to Retinoic Acid Receptor gamma 1 aa 40-57 (N terminal).
Sequence:EMLSPSFRGLGQPDLPKE
(Peptide available asab39773)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS -
PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab5904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human Retinoic Acid Receptor gamma 1 peptide (ab39773). | |
| IHC-P | Use at an assay dependent concentration. | |
| EMSA | Use 1µl for 102 cells. Use 1-2µl. ab5904 has been shown to efficiently super shift the human RAR gamma-1/retinoid X receptor (RXR)/RAR response element complex in gel super shift experiments using a number of different response elements. |
Target
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FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function.
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Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 5916 Human
- Entrez Gene: 19411 Mouse
- Omim: 180190 Human
- SwissProt: P13631 Human
- SwissProt: P18911 Mouse
- Unigene: 1497 Human
- Unigene: 733399 Human
- Unigene: 1273 Mouse
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Alternative names
- NR1B3 antibody
- Nuclear receptor subfamily 1 group B member 3 antibody
- RAR gamma antibody
see all
Images
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Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)All lanes : Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
Lane 1 : HEK293 cell lysate with neutralizing peptide
Lane 2 : HEK293 cell lysate
Predicted band size: 54 kDa
References
This product has been referenced in:
- Joss-Moore L et al. Intrauterine growth restriction transiently delays alveolar formation and disrupts retinoic acid receptor expression in the lung of female rat pups. Pediatr Res 73:612-20 (2013). Read more (PubMed: 23419538) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"