Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
Key features and details
- Rabbit polyclonal to Retinoic Acid Receptor gamma 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Retinoic Acid Receptor gamma 1 antibody -
Description
Rabbit polyclonal to Retinoic Acid Receptor gamma 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit
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Immunogen
Synthetic peptide corresponding to Retinoic Acid Receptor gamma 1 aa 40-57 (N terminal).
Sequence:EMLSPSFRGLGQPDLPKE
(Peptide available asab39773) -
Positive control
- WB: MCF7, BeWo, HaCaT, HepG2 and HEK293 cell lysate. Mouse Mammary Gland and Rat brain tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
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| Notes |
|---|
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WB
1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). |
Target
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Function
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5916 Human
- Entrez Gene: 19411 Mouse
- Entrez Gene: 685072 Rat
- Omim: 180190 Human
- SwissProt: P13631 Human
- SwissProt: P18911 Mouse
- Unigene: 1497 Human
- Unigene: 733399 Human
see all -
Alternative names
- NR1B3 antibody
- Nuclear receptor subfamily 1 group B member 3 antibody
- RAR gamma antibody
see all
Images
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Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)All lanes : Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904) at 1/500 dilution
Lane 1 : MCF7 Nuclear enriched extract lysate
Lane 2 : BeWo Nuclear enriched extract lysate
Lane 3 : HaCaT Nuclear enriched extract lysate
Lane 4 : HepG2 Nuclear enriched extract lysate
Lane 5 : Mouse Mammary Gland tissue lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Predicted band size: 54 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Detection: chemiluminescence.
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Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)All lanes : Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
Lane 1 : HEK293 cell lysate with neutralizing peptide
Lane 2 : HEK293 cell lysate
Predicted band size: 54 kDa -
Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904) at 1/250 dilution + Rat brain tissue lysate at 30 µg
Secondary
Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 0.25 µg/ml
Predicted band size: 54 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?Detection: chemiluminescence.
Datasheets and documents
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Datasheet download
References (2)
ab5904 has been referenced in 2 publications.
- Kadigamuwa C et al. Role of Retinoic Acid Receptor-? in DNA Damage-Induced Necroptosis. iScience 17:74-86 (2019). PubMed: 31255985
- Joss-Moore L et al. Intrauterine growth restriction transiently delays alveolar formation and disrupts retinoic acid receptor expression in the lung of female rat pups. Pediatr Res 73:612-20 (2013). PubMed: 23419538
