Key features and details
- Rabbit polyclonal to Retinoic Acid Receptor gamma 2 - N-terminal
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Retinoic Acid Receptor gamma 2 antibody - N-terminal
See all Retinoic Acid Receptor gamma 2 primary antibodies
DescriptionRabbit polyclonal to Retinoic Acid Receptor gamma 2 - N-terminal
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Purkinje cells, PC-3 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab188000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 50 kDa.|
FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
- Information by UniProt
- NR1B3 antibody
- Nuclear receptor subfamily 1 group B member 3 antibody
- RAR gamma antibody
Anti-Retinoic Acid Receptor gamma 2 antibody - N-terminal (ab188000) + MCF7
Retinoic Acid Receptor gamma 2
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Purkinje cells, labeling Retinoic Acid Receptor gamma 2 with ab188000 at 1/50 dilution.
Immunofluorescent analysis of PFA-fixed/Triton X-100-permeabilized Human cell line PC-3, labeling Retinoic Acid Receptor gamma 2 with ab188000 at 4 µg/ml.
All lanes : Anti-Retinoic Acid Receptor gamma 2 antibody - N-terminal (ab188000)
Lane 1 : HEK293T cell lysate transfected with vector only
Lane 2 : HEK293T cell lysate transfected with Retinoic Acid Receptor gamma 2 (co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)
Predicted band size: 50 kDa
ab188000 has not yet been referenced specifically in any publications.