Overview

  • Product name

    Anti-Retinoic Acid Receptor gamma antibody
    See all Retinoic Acid Receptor gamma primary antibodies
  • Description

    Rabbit polyclonal to Retinoic Acid Receptor gamma
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Zebrafish
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 119 - 439 of Human Retinoic Acid Receptor gamma (NP_000957).

  • Positive control

    • WB: 293T, A431, H1299, HeLaS3, Raji and NIH3T3 cell lysates IF: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • NR1B3 antibody
    • Nuclear receptor subfamily 1 group B member 3 antibody
    • RAR gamma antibody
    • RAR-gamma antibody
    • RARC antibody
    • Rarg antibody
    • RARG_HUMAN antibody
    • Retinoic acid receptor gamma 2 antibody
    • Retinoic acid receptor gamma antibody
    • Retinoic acid receptor, gamma antibody
    see all

Images

  • Anti-Retinoic Acid Receptor gamma antibody (ab97569) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Anti-Retinoic Acid Receptor gamma antibody (ab97569) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 50 kDa



    7.5% SDS PAGE
  • ab97569, at a 1/200 dilution, staining Retinoic Acid Receptor gamma in paraformaldehyde-fixed HeLa cells, by Immunofluorescence analysis. Right image is merged with a DNA probe.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (F9 teratocarcinoma stem cells)
Specification
F9 teratocarcinoma stem cells
Treatment
vehicle control (EtOH) and all-trans retinoic acid (atRA, 1uM, 24hrs)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Mr. Dan Stummer

Verified customer

Submitted Mar 28 2014

Question
Answer

Thank you for your phone call.
DISCOUNT CODE: xxx
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab97569 in ChIP. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

From the WB image sent, it does look like you're getting a strong specific band at 50 kDa, which is the predicted MW for RARG according to Swiss Prot. It's also possible that the higher stronger band is a dimer. Do you have a lane where the MW markers are shown?

http://www.uniprot.org/uniprot/P13631

So since you're seeing the correct MW, is your concern the other background bands other than the 50 kDa band? You may be able to decrease non-specific binding by diluting the primary and secondary antibodies more, changing the blocking buffer or increasing the concentration or incubation time of the block, incubating the primary overnight at 4C, and including tween in the washes (3 x 5 mins). You may also want to run a no primary control to ensure that the secondary isn't binding non-specifically.

You don't have to worry about sending back the antibody. I'm not sure if there really is a problem here, it just seems like the background needs to be cleaned up a bit.

I hope this information is helpful, and I thank you for your cooperation.

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