Key features and details
- Rabbit polyclonal to Retinoic Acid Receptor gamma
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Retinoic Acid Receptor gamma antibody
See all Retinoic Acid Receptor gamma primary antibodies
DescriptionRabbit polyclonal to Retinoic Acid Receptor gamma
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Zebrafish
Recombinant protein fragment corresponding to a region within amino acids 119 - 439 of Human Retinoic Acid Receptor gamma (NP_000957).
- WB: 293T, A431, H1299, HeLaS3, Raji and NIH3T3 cell lysates IF: HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Retinoic & Retinoid
Our Abpromise guarantee covers the use of ab97569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 50 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
- Information by UniProt
- NR1B3 antibody
- Nuclear receptor subfamily 1 group B member 3 antibody
- RAR gamma antibody
Anti-Retinoic Acid Receptor gamma antibody (ab97569) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE
Anti-Retinoic Acid Receptor gamma antibody (ab97569) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 50 kDa
7.5% SDS PAGE
ab97569, at a 1/200 dilution, staining Retinoic Acid Receptor gamma in paraformaldehyde-fixed HeLa cells, by Immunofluorescence analysis. Right image is merged with a DNA probe.
ab97569 has been referenced in 1 publication.
- Chen J et al. Inhibition of cancer stem cell like cells by a synthetic retinoid. Nat Commun 9:1406 (2018). PubMed: 29643385