Validated using a knockout cell line
Recombinant
RabMAb

Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)

Overview

  • Product name
    Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal
    See all Retinoic Acid Receptor gamma primary antibodies
  • Description
    Rabbit monoclonal [EPR2020(N)] to Retinoic Acid Receptor gamma - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Retinoic Acid Receptor gamma aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P13631-2

  • Positive control
    • NCCIT, NTERA2/D1 and HeLa cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF 1/100 - 1/250.

Target

  • Function
    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NR1B3 antibody
    • Nuclear receptor subfamily 1 group B member 3 antibody
    • RAR gamma antibody
    • RAR-gamma antibody
    • RARC antibody
    • Rarg antibody
    • RARG_HUMAN antibody
    • Retinoic acid receptor gamma 2 antibody
    • Retinoic acid receptor gamma antibody
    • Retinoic acid receptor, gamma antibody
    see all

Images

  • All lanes : Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : RARG (Acid Receptor gamma) knockout HAP1 whole cell lysate
    Lane 3 : A375 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab187159 observed at 50 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab187159 was shown to recognize Acid Receptor gamma in wild-type HAP1 cells as signal was lost at the expected MW in RARG (Acid Receptor gamma) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and RARG (Acid Receptor gamma) knockout samples were subjected to SDS-PAGE. Ab187159 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159) at 1/1000 dilution

    Lane 1 : NCCIT cell lysate
    Lane 2 : NTERA2/D1 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa

  • Immunofluorescent analysis of HeLa cells labeling Retinoic Acid Receptor gamma with ab187159 at 1/250 and DAPI staining (blue).

References

ab187159 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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