Anti-Retinoid X Receptor alpha/RXRA antibody (ab227273)
Key features and details
- Rabbit polyclonal to Retinoid X Receptor alpha/RXRA
- Suitable for: WB, IP
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Retinoid X Receptor alpha/RXRA antibody
See all Retinoid X Receptor alpha/RXRA primary antibodies -
Description
Rabbit polyclonal to Retinoid X Receptor alpha/RXRA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment within Human Retinoid X Receptor alpha/RXRA (internal sequence). The exact sequence is proprietary.
Database link: P19793 -
Positive control
- WB: Rat liver tissue lysate; HepG2 whole cell lysate. IP: MCF7 whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/10000. Predicted molecular weight: 51 kDa.
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IP |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/10000. Predicted molecular weight: 51 kDa. |
IP
1/100 - 1/500. |
Target
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Function
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. -
Tissue specificity
Highly expressed in liver, also found in lung, kidney and heart. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain). -
Post-translational
modificationsPhosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6256 Human
- Entrez Gene: 20181 Mouse
- Entrez Gene: 25271 Rat
- Omim: 180245 Human
- SwissProt: P19793 Human
- SwissProt: P28700 Mouse
- SwissProt: Q05343 Rat
- Unigene: 590886 Human
see all -
Alternative names
- FLJ00280 antibody
- FLJ00318 antibody
- FLJ16020 antibody
see all
Images
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Anti-Retinoid X Receptor alpha/RXRA antibody (ab227273) at 1/500 dilution + Rat liver tissue lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa10% SDS-PAGE
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Retinoid X Receptor alpha/RXRA was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell extract with 4 μg ab227273. Western blot was performed from the immunoprecipitate using ab227273 at 1/500 dilution.
Lane 1: Control IgG IP in MCF7 whole cell extract.
Lane 2: ab227273 IP in MCF7 whole cell extract.
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Anti-Retinoid X Receptor alpha/RXRA antibody (ab227273) at 1/5000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa10% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227273 has not yet been referenced specifically in any publications.