Overview

  • Product name

    Anti-Retinoid X Receptor alpha/RXRA antibody
    See all Retinoid X Receptor alpha/RXRA primary antibodies
  • Description

    Goat polyclonal to Retinoid X Receptor alpha/RXRA
  • Host species

    Goat
  • Specificity

    This does not cross-react with either RXR beta or gamma.
  • Tested applications

    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Retinoid X Receptor alpha/RXRA aa 14-28 (N terminal).
    Sequence:

    (C)QVNSSLTSP TGRGSM

  • Positive control

    • Human epidermoid carcinoma A431 lysates.
  • General notes

     This product was previously labelled as Retinoid X Receptor alpha

     

Properties

Applications

Our Abpromise guarantee covers the use of ab24363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 22205725
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
ELISA 1/16000.

Target

  • Function

    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
  • Tissue specificity

    Highly expressed in liver, also found in lung, kidney and heart.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
    Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ00280 antibody
    • FLJ00318 antibody
    • FLJ16020 antibody
    • FLJ16733 antibody
    • MGC102720 antibody
    • NR2B1 antibody
    • Nuclear receptor subfamily 2 group B member 1 antibody
    • OTTHUMP00000022510 antibody
    • Retinoic acid receptor RXR alpha antibody
    • Retinoic acid receptor RXR-alpha antibody
    • Retinoid X nuclear receptor alpha antibody
    • Retinoid X receptor alpha antibody
    • RXR alpha1 antibody
    • Rxra antibody
    • RXRA_HUMAN antibody
    • RXRalpha1 antibody
    see all

Images

  • Anti-Retinoid X Receptor alpha/RXRA antibody (ab24363) at 0.3 µg/ml + Nuclear HeLa lysate at 35 µg

    Predicted band size: 51 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.

    The observed molecular weight corresponds to earlier findings in literature with different antibodies (Solomon et al, J Biol Chem. 1998 Jul 10;273(28):17573-8; PMID: 9651351. 

References

This product has been referenced in:

  • Castella B  et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and V?9Vd2 T cell activation by dendritic cells. Nat Commun 8:15663 (2017). Read more (PubMed: 28580927) »
  • Nickkho-Amiry M  et al. Peroxisome proliferator-activated receptors modulate proliferation and angiogenesis in human endometrial carcinoma. Mol Cancer Res 10:441-53 (2012). WB, IHC-P ; Human . Read more (PubMed: 22205725) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Intestinal tissue)
Antigen retrieval step
None
Permeabilization
No
Specification
Intestinal tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 12 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Gel Running Conditions
Reduced Denaturing (NuPage Bis-Tris 4-12% gradient)
Loading amount
40 µg
Specification
liver
Blocking step
Odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Dr. David Rudnick

Verified customer

Submitted Aug 16 2016

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