Key features and details
- Goat polyclonal to Retinoid X Receptor alpha/RXRA
- Suitable for: WB, ELISA, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Retinoid X Receptor alpha/RXRA antibody
See all Retinoid X Receptor alpha/RXRA primary antibodies
DescriptionGoat polyclonal to Retinoid X Receptor alpha/RXRA
SpecificityThis does not cross-react with either RXR beta or gamma.
Tested applicationsSuitable for: WB, ELISA, Flow Cyt, ICCmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- WB: HeLa and K562 nuclear lysates; ICC: MCF7 cells; Flow Cyt: MCF7 cells.
This product was previously labelled as Retinoid X Receptor alpha
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Retinoic & Retinoid
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab24363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
1 hour primary incubation is recommended for this product.
|Flow Cyt||Use a concentration of 10 µg/ml.|
|ICC||Use a concentration of 10 µg/ml.|
FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
Tissue specificityHighly expressed in liver, also found in lung, kidney and heart.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
modificationsPhosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
- Information by UniProt
- FLJ00280 antibody
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All lanes : Anti-Retinoid X Receptor alpha/RXRA antibody (ab24363) at 0.3 µg/ml
Lane 1 : HeLa nuclear lysate (in RIPA buffer)
Lane 2 : HeLa nuclear lysate (in RIPA buffer) with peptide
Lane 3 : K562 nuclear lysate (in RIPA buffer)
Lane 4 : K562 nuclear lysate (in RIPA buffer) with peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 51 kDa
Observed band size: 55-60 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry analysis of MCF7 cells labelling Retinoid X Receptor alpha/RXRA with ab24363 at 10 µg/mL showing strong nuclear staining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Primary incubation for 1 hour. Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). Actin filaments were stained with phalloidin (red). Nuclear DNA was labelled with DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) labelling Retinoid X Receptor alpha/RXRA with ab24363. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
ab24363 has been referenced in 2 publications.
- Castella B et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and V?9Vd2 T cell activation by dendritic cells. Nat Commun 8:15663 (2017). PubMed: 28580927
- Nickkho-Amiry M et al. Peroxisome proliferator-activated receptors modulate proliferation and angiogenesis in human endometrial carcinoma. Mol Cancer Res 10:441-53 (2012). WB, IHC-P ; Human . PubMed: 22205725