Overview

  • Product name

    Anti-Retinoid X Receptor alpha/RXRA antibody
    See all Retinoid X Receptor alpha/RXRA primary antibodies
  • Description

    Rabbit polyclonal to Retinoid X Receptor alpha/RXRA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Retinoid X Receptor alpha/RXRA conjugated to keyhole limpet haemocyanin.

  • Positive control

    • An induced culture of E. coli
  • General notes

     This product was previously labelled as Retinoid X Receptor alpha

     

Applications

Our Abpromise guarantee covers the use of ab86027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 51 kDa). Tag is 14kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
    • Tissue specificity

      Highly expressed in liver, also found in lung, kidney and heart.
    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR2 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Domain

      Composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
    • Post-translational
      modifications

      Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
      Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ00280 antibody
      • FLJ00318 antibody
      • FLJ16020 antibody
      • FLJ16733 antibody
      • MGC102720 antibody
      • NR2B1 antibody
      • Nuclear receptor subfamily 2 group B member 1 antibody
      • OTTHUMP00000022510 antibody
      • Retinoic acid receptor RXR alpha antibody
      • Retinoic acid receptor RXR-alpha antibody
      • Retinoid X nuclear receptor alpha antibody
      • Retinoid X receptor alpha antibody
      • RXR alpha1 antibody
      • Rxra antibody
      • RXRA_HUMAN antibody
      • RXRalpha1 antibody
      see all

    Images

    • All lanes : Anti-Retinoid X Receptor alpha/RXRA antibody (ab86027) at 1/1000 dilution

      Lane 1 : An un-induced culture of E. coli
      Lane 2 : An induced culture of E. coli

      Lysates/proteins at 1/5000 dilution per lane.

      Secondary
      All lanes : Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size: 51 kDa
      Observed band size: 65 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 45 kDa, 46 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 60 minutes


      Gel concentration 10%Predicted molecular weight of Retinoid X Receptor alpha/RXRA 64.8kDa (50.8kDa + another 14kDa for the tag).

    References

    ab86027 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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