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  1. Link

    retinoid-x-receptor-alpharxra-antibody-epr7106-ab125001.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (14)

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Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
  • Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
  • Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
  • Immunoprecipitation - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
  • Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
  • Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
  • OI-RD Scanning - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
  • Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7106] to Retinoid X Receptor alpha/RXRA
  • Suitable for: WB, IP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

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Secondary
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Knockout
Product image
Human RXRA knockout HCT116 cell line (ab273708)

View more associated products

Overview

  • Product name

    Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]
    See all Retinoid X Receptor alpha/RXRA primary antibodies
  • Description

    Rabbit monoclonal [EPR7106] to Retinoid X Receptor alpha/RXRA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Retinoid X Receptor alpha/RXRA (N terminal).

  • Positive control

    • WB: MCF7, HeLa, K562, RAW 264.7, PC12 and NIH 3T3 cell lysates, Wild-type HCT116 cell lysate. ICC/IF: MCF7 cells and wild-type HCT116 cells. IP: HeLa whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 1.40 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7106
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Retinoic & Retinoid
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Retinoic & Retinoid
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Retanoic and retanoid
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • HRP Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab200782)
    • Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] - BSA and Azide free (ab232472)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human RXRA knockout HCT116 cell line (ab273708)
  • KO cell lysates

    • Human RXRA knockout HCT116 cell lysate (ab275245)
  • Positive Controls

    • K-562 nuclear extract lysate (ab14851)
    • MCF7 nuclear extract lysate (ab14860)
    • NIH/3T3 nuclear extract lysate (ab14874)
    • PC-12 nuclear extract lysate (ab14884)
  • Recombinant Protein

    • Recombinant Human Retinoid X Receptor alpha/RXRA protein (ab82050)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab125001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
IP
1/10 - 1/100.
ICC/IF
Use a concentration of 0.2 µg/ml.

For unpurified use at 1/100 - 1/250 dilution.

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
IP
1/10 - 1/100.
ICC/IF
Use a concentration of 0.2 µg/ml.

For unpurified use at 1/100 - 1/250 dilution.

Application notes
Is unsuitable for Flow Cyt or IHC-P.

Target

  • Function

    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
  • Tissue specificity

    Highly expressed in liver, also found in lung, kidney and heart.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
    Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P19793 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6256 Human
    • Entrez Gene: 20181 Mouse
    • Entrez Gene: 25271 Rat
    • Omim: 180245 Human
    • SwissProt: P19793 Human
    • SwissProt: P28700 Mouse
    • SwissProt: Q05343 Rat
    • Unigene: 590886 Human
    • Unigene: 24624 Mouse
    • Unigene: 108206 Rat
    see all
  • Alternative names

    • FLJ00280 antibody
    • FLJ00318 antibody
    • FLJ16020 antibody
    • FLJ16733 antibody
    • MGC102720 antibody
    • NR2B1 antibody
    • Nuclear receptor subfamily 2 group B member 1 antibody
    • OTTHUMP00000022510 antibody
    • Retinoic acid receptor RXR alpha antibody
    • Retinoic acid receptor RXR-alpha antibody
    • Retinoid X nuclear receptor alpha antibody
    • Retinoid X receptor alpha antibody
    • RXR alpha1 antibody
    • Rxra antibody
    • RXRA_HUMAN antibody
    • RXRalpha1 antibody
    see all

Images

  • Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    All lanes : Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001) at 1/1000 dilution

    Lane 1 : Wild-type HCT116 cell lysate
    Lane 2 : RXRA knockout HCT116 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 51 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab125001 observed at 53 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab125001 was shown to react with Anti-Retinoid X Receptor alpha in HCT 116 wild-type cells in western blot with loss of signal observed in RXRA knockout cell line ab273708 (RXRA knockout cell lysate ab275245). HCT 116 wild-type and RXRA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab125001 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    ab125001 staining Retinoid X Receptor alpha in wild-type HCT116 cells (top panel) and RXRA knockout HCT116 cells (bottom panel) (ab273708). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab125001 at 0.2μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
  • Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    All lanes : Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001) at 1/1000 dilution (purified)

    Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Immunoprecipitation - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

    ab125001 (purified) at 1:20 dilution (0.6μg) immunoprecipitating Retinoid X Receptor alpha/RXRA in HeLa whole cell lysate.

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, 10μg. 
    Lane 2 (+): ab125001 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125001 in HeLa whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

    Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Retinoid X Receptor alpha/RXRA with Purified ab125001 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    All lanes : Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001) at 1/1000 dilution (unpurified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : RAW 264.7 cell lysate
    Lane 5 : PC12 cell lysate
    Lane 6 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 51 kDa

  • OI-RD Scanning - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    OI-RD Scanning - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (14)

Publishing research using ab125001? Please let us know so that we can cite the reference in this datasheet.

ab125001 has been referenced in 14 publications.

  • Pei J  et al. Multi-omics integration identifies key upstream regulators of pathomechanisms in hypertrophic cardiomyopathy due to truncating MYBPC3 mutations. Clin Epigenetics 13:61 (2021). PubMed: 33757590
  • Song Y  et al. A Non-coding HES1 Variant Predisposes Children to Congenital Heart Disease in Chinese Population. Front Cell Dev Biol 9:631942 (2021). PubMed: 33585489
  • Cao H  et al. Retinoid X Receptor a Regulates DHA-Dependent Spinogenesis and Functional Synapse Formation In Vivo. Cell Rep 31:107649 (2020). PubMed: 32433958
  • Zhang S  et al. Electro-Acupuncture Promotes the Differentiation of Endogenous Neural Stem Cells via Exosomal microRNA 146b After Ischemic Stroke. Front Cell Neurosci 14:223 (2020). PubMed: 32792909
  • Liu J  et al. Disrupted hepatic pentose phosphate pathway directly participates in and indirectly promotes CYP3A reduction: A new strategy for CYP3A-mediated drug hepatotoxicity. Br J Pharmacol 177:1538-1555 (2020). PubMed: 31670839
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat liver)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
15 µg
Specification
Rat liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Yu Siong Ho

Verified customer

Submitted Nov 25 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic stem cells)
Permeabilization
Yes - PBS-Triton 0.3%
Specification
embryonic stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 29 2018

Western blot abreview for Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Pig Cell lysate - whole cell (Primary brain endothelial cells)
Gel Running Conditions
Reduced Denaturing (10% resolving gel)
Loading amount
10 µg
Specification
Primary brain endothelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Yu Siong Ho

Verified customer

Submitted Oct 23 2017

Western blot abreview for Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Ovarian granulosa)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Loading amount
500000 cells
Specification
Ovarian granulosa
Blocking step
Odyssey Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Suman Rice

Verified customer

Submitted Oct 19 2015

Immunohistochemistry (Frozen sections) abreview for Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Multiple embryonic tissues (liver, heart, intestin)
Permeabilization
Yes - 0.3% Triton
Specification
Multiple embryonic tissues (liver, heart, intestin
Blocking step
10% Donkey serum + 10% Roche blocking solution (casein-based) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
both snap-frozen unfixed and 4% paraformaldehyde-fixed (pH7.4, 24h)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Yaacov Barak

Verified customer

Submitted Feb 13 2015

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