Name: Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]
Brand: RabMAb
SKU: ab125001
Rating: 5 (4 reviews)

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Primary

Secondary

This product Rabbit Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
WB, IP, ICC/IF

Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

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Product name

Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]
See all Retinoid X Receptor alpha/RXRA primary antibodies
Description

Rabbit monoclonal [EPR7106] to Retinoid X Receptor alpha/RXRA
Host species

Rabbit
Tested applications

Suitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide corresponding to Human Retinoid X Receptor alpha/RXRA (N terminal).
Positive control
- MCF7, HeLa, K562, RAW 264.7, PC12 and NIH 3T3 cell lysates.
General notes

This product was previously labelled as Retinoid X Receptor alpha

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Dissociation constant (K_D)

K_D = 1.40 x 10 ^-12 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7106
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human Retinoid X Receptor alpha/RXRA protein (ab82050)

Our Abpromise guarantee covers the use of ab125001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
IP		1/10 - 1/100.
ICC/IF		1/500. For unpurified use at 1/100 - 1/250.

Application notes

Is unsuitable for Flow Cyt or IHC-P.

Function

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
Tissue specificity

Highly expressed in liver, also found in lung, kidney and heart.
Sequence similarities

Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain

Composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
Post-translational
modifications

Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
Cellular localization

Nucleus.
Target information above from: UniProt accession P19793 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6256 Human
- Entrez Gene: 20181 Mouse
- Entrez Gene: 25271 Rat
- Omim: 180245 Human
- SwissProt: P19793 Human
- SwissProt: P28700 Mouse
- SwissProt: Q05343 Rat
- Unigene: 590886 Human
- Unigene: 24624 Mouse
- Unigene: 108206 Rat
see all
Alternative names
- FLJ00280 antibody
- FLJ00318 antibody
- FLJ16020 antibody
- FLJ16733 antibody
- MGC102720 antibody
- NR2B1 antibody
- Nuclear receptor subfamily 2 group B member 1 antibody
- OTTHUMP00000022510 antibody
- Retinoic acid receptor RXR alpha antibody
- Retinoic acid receptor RXR-alpha antibody
- Retinoid X nuclear receptor alpha antibody
- Retinoid X receptor alpha antibody
- RXR alpha1 antibody
- Rxra antibody
- RXRA_HUMAN antibody
- RXRalpha1 antibody
see all

Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

All lanes : Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001) at 1/1000 dilution (purified)

Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 51 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

ab125001 (purified) at 1:20 dilution (0.6μg) immunoprecipitating Retinoid X Receptor alpha/RXRA in HeLa whole cell lysate.

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, 10μg.
Lane 2 (+): ab125001 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125001 in HeLa whole cell lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Retinoid X Receptor alpha/RXRA with Purified ab125001 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

All lanes : Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001) at 1/1000 dilution (unpurified)

Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : RAW 264.7 cell lysate
Lane 5 : PC12 cell lysate
Lane 6 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 51 kDa
Other - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D

Western blot protocols
Immunoprecipitation protocols
Immunocytochemistry & immunofluorescence protocols

This product has been referenced in:

Shalom-Barak T et al. Ligand-Dependent Corepressor (LCoR) Is a Rexinoid-Inhibited Peroxisome Proliferator-Activated Receptor ?-Retinoid X Receptor a Coactivator. Mol Cell Biol 38:N/A (2018). Read more (PubMed: 29463649) »
Liu L et al. Hepatic Tmem30a Deficiency Causes Intrahepatic Cholestasis by Impairing Expression and Localization of Bile Salt Transporters. Am J Pathol 187:2775-2787 (2017). Read more (PubMed: 28919113) »

See all 4 Publications for this product

Publishing research using ab125001? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (embryonic stem cells)

Permeabilization

Yes - PBS-Triton 0.3%

Specification

embryonic stem cells

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 29 2018

Application

Western blot

Sample

Pig Cell lysate - whole cell (Primary brain endothelial cells)

Gel Running Conditions

Reduced Denaturing (10% resolving gel)

Loading amount

10 µg

Specification

Primary brain endothelial cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Yu Siong Ho

Verified customer

Submitted Oct 23 2017

Application

Western blot

Sample

Human Cell lysate - whole cell (Ovarian granulosa)

Gel Running Conditions

Reduced Denaturing (4-12% Bis-Tris gel)

Loading amount

500000 cells

Specification

Ovarian granulosa

Blocking step

Odyssey Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C

Dr. S Rice

Verified customer

Submitted Oct 19 2015

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Multiple embryonic tissues (liver, heart, intestin)

Permeabilization

Yes - 0.3% Triton

Specification

Multiple embryonic tissues (liver, heart, intestin

Blocking step

10% Donkey serum + 10% Roche blocking solution (casein-based) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

both snap-frozen unfixed and 4% paraformaldehyde-fixed (pH7.4, 24h)

Dr. Yaacov Barak

Verified customer

Submitted Feb 13 2015

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Recombinant Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

Overview

Properties

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Target

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]

Western blot abreview for Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]

Western blot abreview for Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]

Immunohistochemistry (Frozen sections) abreview for Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]