Immunohistochemistry (Frozen sections) abreview for Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Multiple embryonic tissues (liver, heart, intestin)
Permeabilization
Yes - 0.3% Triton
Specification
Multiple embryonic tissues (liver, heart, intestin
Blocking step
10% Donkey serum + 10% Roche blocking solution (casein-based) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
both snap-frozen unfixed and 4% paraformaldehyde-fixed (pH7.4, 24h)

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Dilution
1/100

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: AF555-Donkey anti-rabbit (Invitrogen)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Dr. Yaacov Barak

Verified customer

Submitted Feb 13 2015

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