Overview

  • Product name

    Anti-Retinoid X Receptor alpha/RXRA antibody - N-terminal
    See all Retinoid X Receptor alpha/RXRA primary antibodies
  • Description

    Rabbit polyclonal to Retinoid X Receptor alpha/RXRA - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment within Human Retinoid X Receptor alpha/RXRA (N terminal). The exact sequence is proprietary.
    Database link: P19793

  • Positive control

    • IHC-P: Mouse liver tissue. ICC/IF: HepG2 cells.
  • General notes

     This product was previously labelled as Retinoid X Receptor alpha

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
  • Tissue specificity

    Highly expressed in liver, also found in lung, kidney and heart.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
    Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ00280 antibody
    • FLJ00318 antibody
    • FLJ16020 antibody
    • FLJ16733 antibody
    • MGC102720 antibody
    • NR2B1 antibody
    • Nuclear receptor subfamily 2 group B member 1 antibody
    • OTTHUMP00000022510 antibody
    • Retinoic acid receptor RXR alpha antibody
    • Retinoic acid receptor RXR-alpha antibody
    • Retinoid X nuclear receptor alpha antibody
    • Retinoid X receptor alpha antibody
    • RXR alpha1 antibody
    • Rxra antibody
    • RXRA_HUMAN antibody
    • RXRalpha1 antibody
    see all

Images

  • Paraffin-embedded mouse liver tissue stained for Retinoid X Receptor alpha/RXRA using ab227292 at 1/500 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Retinoid X Receptor alpha/RXRA (green) using ab227292 at 1/1000 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).

References

ab227292 has not yet been referenced specifically in any publications.

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