Key features and details
- Rabbit polyclonal to Retinoid X Receptor alpha/RXRA - N-terminal
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Retinoid X Receptor alpha/RXRA antibody - N-terminal
See all Retinoid X Receptor alpha/RXRA primary antibodies
DescriptionRabbit polyclonal to Retinoid X Receptor alpha/RXRA - N-terminal
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment within Human Retinoid X Receptor alpha/RXRA (N terminal). The exact sequence is proprietary.
Database link: P19793
- IHC-P: Mouse liver tissue. ICC/IF: HepG2 cells.
This product was previously labelled as Retinoid X Receptor alpha
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Retinoic & Retinoid
Our Abpromise guarantee covers the use of ab227292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
Tissue specificityHighly expressed in liver, also found in lung, kidney and heart.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
modificationsPhosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
- Information by UniProt
- FLJ00280 antibody
- FLJ00318 antibody
- FLJ16020 antibody
Paraffin-embedded mouse liver tissue stained for Retinoid X Receptor alpha/RXRA using ab227292 at 1/500 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Retinoid X Receptor alpha/RXRA (green) using ab227292 at 1/1000 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227292 has not yet been referenced specifically in any publications.