Anti-Retinoid X Receptor beta/RXRB antibody (ab221115)
Key features and details
- Rabbit polyclonal to Retinoid X Receptor beta/RXRB
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Retinoid X Receptor beta/RXRB antibody
See all Retinoid X Receptor beta/RXRB primary antibodies -
Description
Rabbit polyclonal to Retinoid X Receptor beta/RXRB -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment corresponding to Human Retinoid X Receptor beta/RXRB aa 53-83.
Sequence:VAGGEQQTPEPEPGEAGRDGMGDSGRDSRSP
Database link: P28702 -
Positive control
- RT-4 cell lysates; Human tonsil lysate; SK-MEL-30 cells.
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General notes
This product was previously labelled as Retinoid X Receptor beta
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab221115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
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| WB | 1/100 - 1/250. Predicted molecular weight: 57 kDa. |
Target
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Relevance
Retinoid X receptors (RXRs) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. The RXR subfamily consists of at least three similar genes, RXR alpha, RXR beta and RXR gamma, all of which control transcription of target genes mediated by retinoids. RXR beta controls expression of many genes that respond to hormones and vitamins, including thyroid hormone, estrogen, retinoids and vitamin D. RXR beta controls a wide array of genes because of its ability to heterodimerize with other hormone receptors including the thyroid hormone receptor (THR), vitamin D receptor (VDR) and retinoic acid receptor (RAR). -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 6257 Human
- Entrez Gene: 20182 Mouse
- Omim: 180246 Human
- SwissProt: P28702 Human
- SwissProt: P28704 Mouse
- Unigene: 388034 Human
- Unigene: 1243 Mouse
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Alternative names
- DAUD I6 antibody
- DAUDI6 antibody
- H 2RIIBP antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor beta/RXRB antibody (ab221115)
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized SK-MEL-30 cells labeling Retinoid X Receptor beta/RXRB with ab221115 at 4 µg/ml (green).
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Western blot - Anti-Retinoid X Receptor beta/RXRB antibody (ab221115)All lanes : Anti-Retinoid X Receptor beta/RXRB antibody (ab221115) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 57 kDa
Protocols
Datasheets and documents
References (0)
ab221115 has not yet been referenced specifically in any publications.
