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  1. Link

    retinoid-x-receptor-betarxrb-antibody-pcrp-rxrb-2b6-ab277104.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)

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Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)
  • Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)
  • Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)
  • Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)
  • Protein Array - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)

Key features and details

  • Mouse monoclonal [PCRP-RXRB-2B6] to Retinoid X Receptor beta/RXRB
  • Suitable for: Protein Array, IHC-P, Flow Cyt, ICC
  • Reacts with: Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6]
    See all Retinoid X Receptor beta/RXRB primary antibodies
  • Description

    Mouse monoclonal [PCRP-RXRB-2B6] to Retinoid X Receptor beta/RXRB
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, IHC-P, Flow Cyt, ICCmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Retinoid X Receptor beta/RXRB.
    Database link: P28702

  • Positive control

    • Flow cyt: U87,and HeLa cells. ICC: HeLa, and K562 cells. IHC-P: Human lymph node tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, 99.9% PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from bioreactor concentrate
  • Clonality

    Monoclonal
  • Clone number

    PCRP-RXRB-2B6
  • Isotype

    IgG2a
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Retinoic & Retinoid
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Retinoic & Retinoid
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Retanoic and retanoid

Associated products

  • Alternative Versions

    • Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Related Products

    • Recombinant Human Retinoid X Receptor beta/RXRB protein (ab82122)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab277104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use 1-2µg for 106 cells.
ICC
Use a concentration of 1 - 2 µg/ml.
Notes
Protein Array
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use 1-2µg for 106 cells.
ICC
Use a concentration of 1 - 2 µg/ml.

Target

  • Function

    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE).
  • Tissue specificity

    Expressed in a variety of tumor cell lines.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P28702 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6257 Human
    • Omim: 180246 Human
    • SwissProt: P28702 Human
    • Unigene: 388034 Human
    • Alternative names

      • DAUD I6 antibody
      • DAUDI6 antibody
      • H 2RIIBP antibody
      • H2RIIBP antibody
      • MGC1831 antibody
      • MHC class I promoter binding protein antibody
      • NR2B2 antibody
      • Nuclear receptor subfamily 2 group B member 2 antibody
      • RCoR 1 antibody
      • RCoR1 antibody
      • Retinoic acid receptor RXR beta antibody
      • Retinoic acid receptor RXR-beta antibody
      • Retinoid X receptor beta antibody
      • RXR beta antibody
      • Rxrb antibody
      • RXRB_HUMAN antibody
      • RXRbeta antibody
      see all

    Images

    • Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)
      Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)

      Immunofluorescence analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Retinoid X Receptor beta/RXRB with ab277104 at 2 µg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).

    • Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)
      Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)

      Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Retinoid X Receptor beta/RXRB using ab277104 at 2 µg / 106  cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)

      Formalin-fixed, paraffin-embedded human lymph node tissue stained for Retinoid X Receptor beta/RXRB using ab277104 at 2 µg/ml in immunohistochemical analysis.

    • Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)
      Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)

      Flow cytometry analysis of PFA-fixed U87 (human glioblastoma-astrocytoma epithelial cell line) cells labeling Retinoid X Receptor beta/RXRB using ab277104 at 2 µg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

    • Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)
      Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)

      Immunofluorescence analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Retinoid X Receptor beta/RXRB with ab277104 at 2 µg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).

    • Protein Array - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)
      Protein Array - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] (ab277104)

      Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277104.


      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab277104? Please let us know so that we can cite the reference in this datasheet.

    ab277104 has not yet been referenced specifically in any publications.

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