Product nameAnti-Retinoid X Receptor gamma antibody
See all Retinoid X Receptor gamma primary antibodies
DescriptionRabbit polyclonal to Retinoid X Receptor gamma
Tested applicationsSuitable for: IHC-Fr, IHC - Wholemount, ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Hamster, Human
Synthetic peptide within Mouse Retinoid X Receptor gamma aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P28705
- Breast carcinoma and prostate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Retinoic & Retinoid
Our Abpromise guarantee covers the use of ab15518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC - Wholemount||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
- Information by UniProt
- MGC109416 antibody
- NR2B3 antibody
- Nuclear receptor subfamily 2 group B member 3 antibody
Anti-Retinoid X Receptor gamma antibody (ab15518) at 1/25 dilution + NIH3T3 cell lysate
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
IHC - Wholemount of Chicken inner ear tissue labelling Retinoid X Receptor gamma with ab15518. Sample was incubated with primary antibody (1/500 in 0.2% Triton-X in PBS) for 12 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
ab15518 staining Retinoid X Receptor gamma in breast carcinoma by Immunohistochemistry (FFPE-sections).
Immunostaining of Retinoid X receptor gamma in adult mouse retina using ab15518. Visible are the outer nuclear layer (top) and inner nuclear layer (bottom) of a adult mouse retina. Retionoid X receptor gamma staining was observed in the cone photoreceptors in the outer nuclear layer and also in most if not all cells of the inner nuclear layer. Please see abreview for protocol details.
This product has been referenced in:
- Rambow F et al. Toward Minimal Residual Disease-Directed Therapy in Melanoma. Cell 174:843-855.e19 (2018). Read more (PubMed: 30017245) »
- Jean-Charles N et al. Identification and characterization of early photoreceptor cis-regulatory elements and their relation to Onecut1. Neural Dev 13:26 (2018). Read more (PubMed: 30466480) »