Overview

  • Product name
    Anti-Retinoid X Receptor gamma antibody
    See all Retinoid X Receptor gamma primary antibodies
  • Description
    Rabbit polyclonal to Retinoid X Receptor gamma
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, IHC - Wholemount, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Hamster, Human
  • Positive control
    • Breast carcinoma and prostate.

Properties

Applications

Our Abpromise guarantee covers the use of ab15518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200.
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF 1/100.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC109416 antibody
    • NR2B3 antibody
    • Nuclear receptor subfamily 2 group B member 3 antibody
    • OTTHUMP00000060418 antibody
    • Retanoic X receptor gamma antibody
    • Retinoic acid receptor RXR gamma antibody
    • Retinoic acid receptor RXR-gamma antibody
    • Retinoid X receptor gamma antibody
    • RXR G antibody
    • RXR gamma antibody
    • RXRC antibody
    • Rxrg antibody
    • RXRG_HUMAN antibody
    • RXRgamma antibody
    see all

Images

  • IHC - Wholemount of Chicken inner ear tissue labelling Retinoid X Receptor gamma with ab15518. Sample was incubated with primary antibody (1/500 in 0.2% Triton-X in PBS) for 12 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab15518 staining Retinoid X Receptor gamma in breast carcinoma by Immunohistochemistry (FFPE-sections).
  • Immunostaining of Retinoid X receptor gamma in adult mouse retina using ab15518. Visible are the outer nuclear layer (top) and inner nuclear layer (bottom) of a adult mouse retina. Retionoid X receptor gamma staining was observed in the cone photoreceptors in the outer nuclear layer and also in most if not all cells of the inner nuclear layer. Please see abreview for protocol details.

    See Abreview

  • Anti-Retinoid X Receptor gamma antibody (ab15518) at 1/25 dilution + NIH3T3 cell lysate

    Predicted band size: 51 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Rambow F  et al. Toward Minimal Residual Disease-Directed Therapy in Melanoma. Cell 174:843-855.e19 (2018). Read more (PubMed: 30017245) »
  • de la Fuente AG  et al. Vitamin D receptor-retinoid X receptor heterodimer signaling regulates oligodendrocyte progenitor cell differentiation. J Cell Biol 211:975-85 (2015). Read more (PubMed: 26644513) »
See all 6 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain tissue lysate)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (3-8% Bis-Tris Gels)
Loading amount
10 µg
Specification
Whole brain tissue lysate
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Mar 14 2017

Application
Western blot
Loading amount
50000 cells
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (Schwann cells)
Specification
Schwann cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 27 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Heat-inactivated normal donkey serum in 0.05% PBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Mouse, whole brain sections)
Specification
Mouse, whole brain sections
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 07 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Chicken Tissue (Inner Ear Tissue)
Specification
Inner Ear Tissue

Abcam user community

Verified customer

Submitted Jun 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (adult retina)
Specification
adult retina
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton-x100
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Feb 19 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (RXRg expression in U2OS)
Specification
RXRg expression in U2OS
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton X-100
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C

Dr. Mahesh Mathrubutham

Verified customer

Submitted Feb 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO K1 cells expressing Human Retinoid X Receptor)
Specification
CHO K1 cells expressing Human Retinoid X Receptor
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton X-100
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C

Dr. Mahesh Mathrubutham

Verified customer

Submitted Sep 05 2007

Answer

I'm very sorry to hear you are experiencing problems with our RXR and RAR antibodies, thank you for sending us an image of your blots, I understand your problem. The difference in MW can be slightly different between predicted and detected MW and we observe this in numerous cases, especially between different species and in cancer cell lines where a high number of passages can induce a change in a number of proteins. Furthermore, the MW markers are not very specific and can migrate at different sizes in different gels, they should be used to monitor the progress of runs and make valid gel-to-gel comparisons, but can only estimate molecular weights and identify proteins in polyacrylamide gels. The best way to find out if the band you detect is the correct protein is by running a positive control along your samples. For RAR antibodies ab9639, ab9638 and ab9640 the positive control is rat hippocampal homogenate, for RXR alpha ab12014 it is MCF7 nuclear extract, for ab2815 a transfected Sf9 cell line was used unfortunately and for RXR gamma breast carcinoma and prostate tissue (nuclear lysates) were used. I would also make sure that the protease inhibitors in the lysis buffer are all fresh and not old and samples are kept on ice at all times prior to mixing with reducing loading buffer, and please make sure you do nuclear extraction of the proteins. Finally, the multiple bands you are observing (especially RAR alpha, RXR alpha and RXR beta) could be due to too much blocking (and maybe over-exposing the blots for RXR beta). I can certainly take a closer look at your protocol and hopefully make some suggestions which could help you, please do not hesitate to click on the link below which will take you to a questionnaire to help you compile all your protocol information rapidly, I hope the information above will help you, please let me know if I can be of further assistance,

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